Glycerol-enhanced detection of a preferential structure latent in unstructured 1SS-variants of lysozyme

Biopolymers
Yasuo NodaShin-ichi Segawa

Abstract

Four species of 1SS-varinats of lysozyme were almost unstructured in water, judged from their near-UV CD and (1) H-(15) N-HSQC spectra. Some preferential structure might exist in such a disordered state, but the population of molecules in such a conformation must have been too small to be detected by spectroscopic methods. Indeed, our previous study showed that the addition of 30% glycerol induced the unstructured 2SS-variant of lysozyme to form a native-like structure. To extend this method to more disordered proteins, we attempted to detect some preferential structure latent in unstructured 1SS-variants by the glycerol-enhanced detection. Only in one molecular species of the four 1SS-variants, 1SS[6-127] containing a single disulfide bridge of Cys6-Cys127, a preferential structure was found in the presence of 50% glycerol. It was detected by near-UV CD measurements and the H/D exchange method combined with the NMR spectroscopy. The glycerol-induced structure in 1SS[6-127] was not localized only in the vicinity of Cys6-Cys127, and largely protected regions distributed themselves among A-, B-, and C-helices and Ile55 and Leu56. It was similar to the glycerol-induced structure in 2SS[6-127, 64-80] containing two disulfide bridge...Continue Reading

References

Apr 1, 1995·Protein Science : a Publication of the Protein Society·Y Z ZhangH Roder
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Jul 7, 1998·Journal of Molecular Biology·L C Wu, P S Kim
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y QuD W Bolen
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·T KnubovetsA M Klibanov
Mar 19, 1999·Protein Science : a Publication of the Protein Society·A YokotaS Segawa
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·G ChakshusmathiR Varadarajan
Jul 9, 1999·Journal of Molecular Biology·B van den BergC M Dobson
Feb 14, 2002·Journal of the American Chemical Society·Qin ZouKenneth P Murphy
Apr 23, 2002·Nature Structural Biology·Masaru HoshinoYuji Goto
Sep 3, 2005·Chembiochem : a European Journal of Chemical Biology·Emily S CollinsHarald Schwalbe
Jun 30, 2007·The Journal of Chemical Physics·Raffaele SinibaldiPaolo Mariani
Apr 20, 1998·Angewandte Chemie·Christopher M DobsonMartin Karplus

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