PMID: 8584435Jan 1, 1996Paper

Glycerol permeability of mutant aquaporin 1 and other AQP-MIP proteins: inhibition studies

Pflügers Archiv : European journal of physiology
L AbramiP Ripoche

Abstract

In a recent work, we showed that the aquaporins 1 (AQP1) are permeable to certain small solutes such as glycerol. Here, we have further investigated the permeation pathway of glycerol through human AQP1 (hAQP1) by the use of mutants (C189S, H180A, H209A) and inhibitors such as P-chloromercuribenzene sulphonate (pCMBS), CuSO4 or phloretin, in comparison with other AQP-MIP (where MIP denotes major intrinsic protein) proteins: hAQP2, plant water channel gammaTIP and bacterial glycerol permease facilitator, GlpF. Glycerol movements were measured in Xenopus laevis oocytes. Apparent glycerol permeability coefficients (P'gly) were calculated from the rates of oocyte swelling upon exposure to an isoosmotic medium containing an inwardly directed gradient of glycerol and from [3H]glycerol uptake measurements. Similar P'gly values were obtained for hAQP1 and hAQP2 6 to 8 times greater than control indicating that hAQP2 also transports glycerol. P'gly of hAQP2-injected oocytes was pCMBS and CuSO4 sensitive. In contrast, the P'gly value of gammaTIP was close to that of control, indicating that gammaTIP does not transport glycerol. The hAQP1-C189S, -H180A and -H209A mutants gave P'gly values similar to those obtained for wild hAQP1, indicati...Continue Reading

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Citations

Mar 20, 2012·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Daniel WescheNine V A M Knoers
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Christophe Maurel
May 25, 2010·Journal of Nutritional Science and Vitaminology·Mitsuyoshi MiyataKazunaga Yazawa
Jun 30, 2000·Biochemical and Biophysical Research Communications·Z Han, R V Patil
Sep 18, 2007·Protein Expression and Purification·Maria NyblomKristina Hedfalk
Jul 18, 2006·Biochimica Et Biophysica Acta·Cécile Héliès-ToussaintAlain Grynberg
Jul 27, 1999·The Plant Journal : for Cell and Molecular Biology·A BielaR Kaldenhoff
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Jul 27, 1999·The Journal of Biological Chemistry·T Zeuthen, D A Klaerke
Jul 26, 2018·Frontiers in Physiology·Giuseppe CalamitaChristine Delporte
Apr 27, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·A S VerkmanY Song
Nov 7, 2018·Pflügers Archiv : European journal of physiology·Oliver H Wittekindt, Paul Dietl
May 20, 1998·Biochemical and Biophysical Research Communications·W JenqG Ramirez
Sep 23, 1997·Biochemical and Biophysical Research Communications·R V PatilM B Wax

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