Glycerol production by fermenting yeast cells is essential for optimal bread dough fermentation

PloS One
Elham AslankoohiKevin J Verstrepen

Abstract

Glycerol is the main compatible solute in yeast Saccharomyces cerevisiae. When faced with osmotic stress, for example during semi-solid state bread dough fermentation, yeast cells produce and accumulate glycerol in order to prevent dehydration by balancing the intracellular osmolarity with that of the environment. However, increased glycerol production also results in decreased CO2 production, which may reduce dough leavening. We investigated the effect of yeast glycerol production level on bread dough fermentation capacity of a commercial bakery strain and a laboratory strain. We find that Δgpd1 mutants that show decreased glycerol production show impaired dough fermentation. In contrast, overexpression of GPD1 in the laboratory strain results in increased fermentation rates in high-sugar dough and improved gas retention in the fermenting bread dough. Together, our results reveal the crucial role of glycerol production level by fermenting yeast cells in dough fermentation efficiency as well as gas retention in dough, thereby opening up new routes for the selection of improved commercial bakery yeasts.

References

Sep 24, 1982·Science·P H YanceyG N Somero
Feb 6, 1998·FEMS Microbiology Reviews·E Nevoigt, U Stahl
Nov 9, 2000·Letters in Applied Microbiology·P V Attfield, S Kletsas
Feb 13, 2001·FEMS Microbiology Letters·R Hirasawa, K Yokoigawa
Jun 1, 2002·Microbiology and Molecular Biology Reviews : MMBR·Stefan Hohmann
Jul 6, 2006·Applied and Environmental Microbiology·Brigitte CambonSylvie Dequin
Aug 4, 2006·Applied and Environmental Microbiology·Elke NevoigtGregory Stephanopoulos
Sep 19, 2007·Methods in Enzymology·Stefan HohmannBodil Nordlander
Jan 29, 2008·International Journal of Food Microbiology·Carmela BellochAmparo Querol
May 25, 2010·Journal of Food Science·Lien-Te YehTzou-Chi Huang
Sep 24, 2013·Applied and Environmental Microbiology·Elham AslankoohiKevin J Verstrepen

❮ Previous
Next ❯

Citations

Aug 24, 2017·FEMS Microbiology Reviews·Maria C DzialoKevin J Verstrepen
Jul 2, 2020·Ecology and Evolution·Roberto F NespoloFrancisco Bozinovic
Sep 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Nore StruyfChristophe M Courtin
Dec 3, 2020·Frontiers in Genetics·Caitlin LahueCaiti Smukowski Heil
Jun 10, 2017·Microbiology Spectrum·Joëlle DupontMarc-André Selosse
Aug 12, 2021·Comprehensive Reviews in Food Science and Food Safety·Patricia R PereiraVania M F Paschoalin
Sep 16, 2021·Critical Reviews in Food Science and Nutrition·Luc De VuystSimon Van Kerrebroeck

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
rheology

Software Mentioned

Risograph
SAS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.