Glycerol suppresses proliferation of rat hepatocytes and human HepG2 cells

The Journal of Surgical Research
Nozomu SugiyamaJ Rozga

Abstract

In fulminant hepatic failure (FHF), the ability of surviving hepatocytes to proliferate is diminished. Therefore, it is important that medical therapy cause no further impairment of liver regeneration. In FHF, intracranial hypertension secondary to brain edema is the most common cause of brain injury and death and glycerol is used in some countries to treat this complication. Glycerol has been long known to suppress the growth of various cell types. We therefore decided to examine the effect of glycerol on hepatocyte proliferation in vitro and in vivo in rats subjected to partial (2/3) hepatectomy. Additionally, we investigated the effect of glycerol on the proliferation of HepG2 cells. Mitogen-induced primary rat hepatocytes were cultured in a hormonally defined Dulbecco's modified Eagle's medium containing increasing amounts of glycerol (0.5, 1.0, 2.0, 4.0%). HepG2 cells were cultured in minimal essential medium/10% FBS. After 2 days, HepG2 cells were exposed to glycerol (1.0-2.0-4.0%) and harvested after 48 h. Control dishes contained no glycerol. Cell proliferation was measured by the incorporation of [(3)H]thymidine and/or bromodeoxyuridine (BrdU). In vivo, Sprague-Dawley rats were subjected to standard partial 2/3 hepatec...Continue Reading

References

Sep 1, 1977·Journal of Microscopy·F Franks
Jun 1, 1975·Cell Differentiation·H D Preisler, G Lyman
Jun 7, 1975·British Medical Journal·C O RecordR Williams
Feb 19, 1976·Biochemical and Biophysical Research Communications·H W DetrichR C Williams
Dec 1, 1992·British Journal of Clinical Pharmacology·K R Lees
Jan 1, 1991·Life Sciences·J P Wiebe, C J Dinsdale
Jul 1, 1988·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·D J DoolittleC K Lee
Jan 1, 1988·The International Journal of Biochemistry·S Yamamoto, K B Storey
May 1, 1966·American Journal of Ophthalmology·D K McCurdyH G Scheie
Mar 1, 1982·Journal of Neurosurgery·S L Wald, R L McLaurin
Jan 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J H HoofnagleN Ascher
Feb 18, 1998·Mutation Research·J H McGeeR D Sofia
Jul 27, 2001·The Journal of Surgical Research·T MizuguchiJ Rozga

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Citations

Aug 12, 2014·Pediatric Research·Christopher M Traudt, Sandra E Juul
Mar 29, 2014·Journal of Cellular Physiology·Ana SernaMiriam Echevarría
Jan 12, 2016·Channels·Ana Galán-CoboMiriam Echevarría
Oct 20, 2005·Journal of Bioscience and Bioengineering·Tomokatsu HongoHiroyuki Honda
Mar 4, 2017·European Journal of Nutrition·Alejo M CapiglioniMaría de Luján Alvarez
Apr 16, 2020·Environmental Science and Pollution Research International·Amira Abugomaa, Mohamed Elbadawy
Oct 17, 2018·Journal of Biomaterials Science. Polymer Edition·Jeong Eun SongGilson Khang
Sep 11, 2019·Materials Science & Engineering. C, Materials for Biological Applications·Mathilde FenelonJean-Christophe Fricain

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