Glycerol utilization by Rhizobium leguminosarum requires an ABC transporter and affects competition for nodulation

Microbiology
Hao DingM Hynes

Abstract

Plasmid curing has shown that the ability to use glycerol as a carbon source is plasmid-encoded in Rhizobium leguminosarum. We isolated the locus responsible for glycerol utilization from plasmid pRleVF39c in R. leguminosarum bv. viciae VF39. This region was analyzed by DNA sequencing and mutagenesis. The locus encompasses a gene encoding GlpR (a DeoR regulator), genes encoding an ABC transporter, and genes glpK and glpD, encoding a kinase and dehydrogenase, respectively. All the genes except the regulatory gene glpR were organized into a single operon, and were required for growth on glycerol. The glp operon was strongly induced by both glycerol and glycerol 3-phosphate, as well as by pea seed exudate. GlpR repressed the operon in the absence of inducer. Mutation of genes encoding the ABC transporter abolished all transport of glycerol in transport assays using radiolabelled glycerol. This confirms that, unlike in other organisms such as Escherichia coli and Pseudomonas aeruginosa, which use facilitated diffusion, glycerol uptake occurs by an active process in R. leguminosarum. Since the glp locus is highly conserved in all sequenced R. leguminosarum and Rhizobium etli strains, as well as in Sinorhizobium spp. and Agrobacteriu...Continue Reading

References

Feb 1, 1976·Canadian Journal of Microbiology·A Arias, G Martinez-Drets
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1990·Methods in Enzymology·C M Stoscheck
Jan 1, 1985·Annual Review of Microbiology·M D Stowers
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J T Mulligan, S R Long
Sep 1, 1973·Journal of Bacteriology·W B Freedberg, E C Lin
Sep 1, 1974·Journal of General Microbiology·J E Beringer
Jul 26, 1968·Biochemical and Biophysical Research Communications·Y SannoE C Lin
Jan 1, 1981·Nature·G B Ruvkun, F M Ausubel
Feb 1, 1993·Journal of General Microbiology·L BeijerL Rutberg
Jul 1, 1996·Molecular Plant-microbe Interactions : MPMI·W R StreitD A Phillips
Jul 1, 1997·Molecular Plant-microbe Interactions : MPMI·A SchlüterU B Priefer
Oct 6, 1998·Applied and Environmental Microbiology·D A PhillipsC M Joseph
Jul 31, 2004·Annual Review of Phytopathology·Eric B Nelson
Oct 27, 2004·Archives of Microbiology·Christopher K YostMichael F Hynes
Apr 28, 2006·Genome Biology·J Peter W YoungJulian Parkhill
Mar 6, 2007·Journal of Bacteriology·Nathan J Poysti, Ivan J Oresnik
Mar 17, 2009·Applied and Environmental Microbiology·Sonia BañosPaloma Liras

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Citations

Aug 6, 2014·Canadian Journal of Microbiology·Barney A Geddes, Ivan J Oresnik
Nov 12, 2014·Molecular Plant-microbe Interactions : MPMI·Barney A GeddesIvan J Oresnik
Feb 19, 2013·Systematic and Applied Microbiology·Ernesto Ormeño-Orrillo, Esperanza Martínez-Romero
Jul 19, 2012·Molecular Microbiology·Catharine E WhiteTurlough M Finan
May 28, 2015·Archives of Microbiology·Vera I SafronovaIgor A Tikhonovich
Jul 21, 2012·Plasmid·Martha G López-GuerreroEsperanza Martínez-Romero
Aug 16, 2016·Journal of Industrial Microbiology & Biotechnology·Nikolay VassilevMaria Vassileva
Oct 22, 2016·Environmental Microbiology·Timothy J WilliamsRicardo Cavicchioli
Mar 27, 2020·Genes·Ginaini Grazielli Doin de MouraDelphine Capela
Mar 1, 2019·Frontiers in Microbiology·Ala Eddine Cherni, Xavier Perret
Feb 3, 2021·Journal of Bacteriology·Raphael LedermannPhilip S Poole
Jan 12, 2022·MSystems·Carolin C M SchultePhilip S Poole

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