Glycerolipid biosynthesis in isolated rat intestinal epithelial cells.

Canadian Journal of Biochemistry
P J O'Doherty, A Kuksis

Abstract

Intestinal epithelial cells were prepared from fasted rats by dispersion with collagenase (EC 3.4.24.3). The structural and metabolic integrity of the cells was verified by electron microscopy, a high percentage of Trypan Blue exclusion, a low degree of release of lactate dehydrogenase (EC 1.1.1.27) in the medium, and by the retention of sensitivity to agents known to modify metabolic and transport activity in everted sacs of intestinal mucosa. The isolated intestinal epithelial cells were used to study glycerolipid biosynthesis from glucose, glycerol, 2-monoacylglycerol, and free fatty acids. The cells actively incorporated the labeled precursors into glycerolipids without specific cofactor requirements. Addition of fatty acids stimulated the incorporation of both glucose and glycerol into triacylglycerols and glycerophospholipids, the greatest effect being observed with palmitate. The stimulation of monoacylglycerol acylation appeared to depend on both the nature of the monoacylglycerol and fatty acid supplied. Stereospecific analyses of the diacylglycerols formed from 2-monoacylglycerols and free fatty acids showed that 1,2-diacyl-sn-glycerols (62-70%) were the major and that 2,3-diacyl-sn-glycerols (30-38%) the minor interm...Continue Reading

Citations

Jan 1, 1986·Comparative Biochemistry and Physiology. A, Comparative Physiology·H CarlierJ S Hugon
Dec 1, 1979·Environmental Health Perspectives·A KuksisA G Hoffman
Feb 1, 1977·Archives of Biochemistry and Biophysics·S J StohsS Orrenius
Jan 1, 1996·Progress in Lipid Research·R Lehner, A Kuksis
Apr 18, 1980·Biochimica Et Biophysica Acta·C M Lok, D J van der Horst
Dec 1, 1986·Environmental Research·R M Shayiq, A M Kidwai

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