PMID: 9187307May 17, 1997Paper

Glycerolipid metabolizing enzymes in rat ventricle and in cardiac myocytes

Biochimica Et Biophysica Acta
E M Swanton, E D Saggerson

Abstract

1. The properties and subcellular distribution of phosphatidate phosphohydrolase (PAP) were studied in rat heart. A Mg2(+)-activated activity (PAP1) which was inhibited by N-ethylmaleimide was found mainly in a 105,000 x g soluble fraction. Isolation of the membranes in a medium containing KCl increased the proportion of PAP1 that was associated. Translocation of PAP1 from these membranes occurred on subsequent incubation in a low-ionic strength medium from which KCI was omitted. Incubation of cardiac myocytes with palmitate promoted translocation of PAP activity to cellular membranes. A second activity which was insensitive to N-ethylmaleimide (PAP2) was found in the 105,000 x g membrane fraction. PAP2 was inhibited by concentrations of Mg2+ known to occur in ischaemia. Specific activities of PAP1 and PAP2 in ventricle muscle homogenates were similar. The specific activity of PAP2 in homogenates of cardiac myocytes was only 42% of that in homogenates of ventricle muscle. 2. A glycerolphosphate acyltransferase (GPAT) activity with properties similar to the GPAT found in microsomes from liver or adipose tissue was enriched in the sarcoplasmic reticulum fraction from ventricle muscle. This GPAT had a significantly higher K(m) for...Continue Reading

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Citations

Dec 4, 2003·Biochimica Et Biophysica Acta·Tal M Lewin, Rosalind A Coleman
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·S CasesR V Farese
Aug 24, 2012·Plant Physiology·Panneerselvam VijayarajRam Rajasekharan
Aug 20, 2010·Journal of Lipid Research·Biao LuKenneth R Feingold
Nov 8, 2011·Journal of Lipid Research·Bernard P C KokDavid N Brindley
Oct 23, 2013·Chemistry and Physics of Lipids·Edgard M MejiaGrant M Hatch
Feb 11, 2003·The Journal of Biological Chemistry·Jingsong CaoYuguang Shi
Oct 31, 2008·American Journal of Physiology. Cell Physiology·Lauren W CollisonChristopher A Jolly
Jan 28, 2010·American Journal of Physiology. Endocrinology and Metabolism·David N BrindleyJason R B Dyck
Jul 6, 2000·Current Opinion in Lipidology·R V FareseS J Smith
Jun 26, 2003·American Journal of Physiology. Endocrinology and Metabolism·John F LockwoodYuguang Shi
Dec 5, 2002·American Journal of Physiology. Endocrinology and Metabolism·Laura L AtkinsonGary D Lopaschuk
Sep 6, 2002·Biochimica Et Biophysica Acta·Chang-Hua YuNaranjan S Dhalla

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