Glyceroneogenesis: an unexpected metabolic pathway for glutamine in Schistosoma mansoni sporocysts

Molecular and Biochemical Parasitology
Naji KhayathColette Dissous

Abstract

To date, glyceroneogenesis has only been described in mammals but we demonstrate in this paper that it could exist in the invertebrate Schistosoma mansoni, the parasitic helminth transmitted by fresh water molluscs and responsible for the major human endemic disease, schistosomiasis. Glyceroneogenesis is a phosphoenolpyruvate carboxykinase (PEPCK)-dependent process by which glycerol can be produced from precursors like glutamine and therefore represents a truncated gluconeogenic pathway. We have previously demonstrated the possible central role of glutamine in mollusc-schistosome interactions. In this work, we show that glutamine effectively promotes in vitro survival and protein synthesis in sporocysts, the intramolluscan larval stages of S. mansoni, possibly through its role as an energy source. However, the demonstration that PEPCK is massively expressed in these larval forms as compared to adult parasites, together with the observation that 3-mercaptopicolinate, a specific inhibitor of PEPCK, significantly reduces the effect of glutamine on sporocyst growth, suggest that glutamine could also be used for glucose or glycerol production. Results of [14C] glutamine incorporation confirmed that neosynthesis of glucose and mainly...Continue Reading

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Citations

Jun 1, 2010·Parasitology Research·Javier SotilloRafael Toledo
Jul 29, 2009·The Journal of Biological Chemistry·Jianqi YangRichard W Hanson
Jul 27, 2007·International Journal for Parasitology·Yutao YanColette Dissous
Jun 26, 2007·Biochemical and Biophysical Research Communications·Fernanda LudolfGuilherme Oliveira
Oct 13, 2020·PLoS Neglected Tropical Diseases·Alessandra GuidiGiovina Ruberti

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