PMID: 8971734Dec 1, 1996Paper

Glycine does not reverse the inhibitory actions of ethanol on NMDA receptor functions in cerebellar granule cells

Naunyn-Schmiedeberg's Archives of Pharmacology
G CebersS Liljequist

Abstract

The effects of ethanol and/or glycine on NMDA-induced enhancement of cytoplasmic free Ca2+ concentrations ([Ca2+]i), 45Ca2+ influx, 4-b-[3H]phorbol-12,13-dibutyrate ([3H]PDBu) binding, and neuronal necrosis in cultured rat cortical and cerebellar granule neurons were examined. Using microfluorimetric techniques in combination with rapid perfusion of single brain neurons, we found that glycine (10 microM) was a necessary co-agonist for NMDA-induced depolarization in cerebellar granule cells. In contrast, depolarization with NMDA in cortical cells was observed even without the addition of exogenous glycine as well as in the absence or presence of 1 mM MgCl2. Ethanol (50 mM) inhibited the effects of NMDA in some, but not all, neurons indicative of the existence of ethanol-sensitive and ethanol-insensitive cortical and cerebellar granule neurons. In studies performed in monolayers of cortical and cerebellar granule cells, we observed that the presence of glycine (10 microM) was a necessary prerequisite to unmask inhibitory actions of ethanol on 45Ca2+ influx induced by NMDA. In another set of experiments, we noted that NMDA-induced stimulation of [3H]PDBu binding to monolayers of intact cerebellar granule cells was inhibited by eth...Continue Reading

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Citations

Jul 30, 1997·European Journal of Pharmacology·J Kotlinska, S Liljequist
Jul 16, 1999·Neurochemistry International·K WirknerP Illes
Jul 22, 1998·Progress in Neurobiology·C L FaingoldA Riaz
Mar 2, 1999·British Journal of Pharmacology·C AllgaierP Illes
Jan 23, 1998·Alcoholism, Clinical and Experimental Research·V M Moraes Ferreira, G S Morato

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