Glycine-induced cryotransformation of plasmids into Bacillus anthracis

Journal of General Microbiology
A S StepanovB N Ilyashenko

Abstract

Different cloning vectors (pC194, pBC16, pUB110, pBD10, pBD8, pAM beta 1) and Bacillus anthracis plasmid pX02 were introduced into B. anthracis by a transformation method. To induce an artificial competence state for uptake of isolated plasmid DNA, the cultures were treated with glycine, to reduce cross-linking of peptidoglycan, followed by freezing and thawing. The procedure is extremely rapid and relatively efficient (maximum transformation efficiency about 10(3) c.f.u. per micrograms DNA) and allows different cloning vectors with molecular masses ranging from 1.8 to 17.7 MDa to be introduced into B. anthracis.

Citations

Dec 1, 2009·Applied Microbiology and Biotechnology·Trond Erik Vee Aune, Finn Lillelund Aachmann
Jul 19, 2011·Journal of Bioenergetics and Biomembranes·Toshihede MatsunoIsao Yumoto
Dec 12, 2012·Applied and Environmental Microbiology·Quan LuoAlexander Steinbüchel
Jul 6, 2006·Journal of Microbiological Methods·Nathalie TurgeonCaroline Duchaine
Apr 14, 2016·The Journal of Membrane Biology·Tadej Kotnik, James C Weaver
Apr 3, 1998·The Journal of Veterinary Medical Science·T SekizakiS Takai
Jan 16, 2019·Microbiology Resource Announcements·Amy S GargisDavid Sue

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