Glycine intravenous donor preconditioning is superior to glycine supplementation to low-potassium dextran flush preservation and improves graft function in a large animal lung transplantation model after 24 hours of cold ischemia

The Journal of Thoracic and Cardiovascular Surgery
Bernhard GohrbandtMartin Strueber

Abstract

The potential role of glycine in combination with standard lung preservation with low-potassium dextran solution in lung ischemia-reperfusion injury has not been investigated in a preclinical porcine transplant model. In a control group (n = 6), donor lungs were flushed with 1 liter of low-potassium dextran solution. In a second group (LPD-glyc, n = 6), low-potassium dextran solution was supplemented with 3.75 g of glycine. In a third group (IV-glyc, n = 6), donor preconditioning was performed by intravenous administration of 3.75 g glycine 1 hour before low-potassium dextran preservation. Grafts were stored in low-potassium dextran at 4 degrees C for 24 hours. Posttransplant graft function was assessed throughout a 7-hour observation period. In the control group, 2 recipients died of right-sided heart failure caused by severe ischemia-reperfusion injury. All animals of the glycine groups survived the entire observation period. Pulmonary vascular resistance remained significantly (P < .01) lower in both glycine groups when compared with controls. At the end of the observation period pulmonary vascular resistance in the control group was higher (P < .01) compared with the glycine groups (1310 +/- 319 dyn x sec x cm(-5) vs 879 +/...Continue Reading

References

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Citations

Feb 22, 2012·Interactive Cardiovascular and Thoracic Surgery·Sebastian-Patrick SommerRainer G Leyh
Oct 7, 2008·International Immunopharmacology·Miriam S Giambelluca, Oscar A Gende
May 30, 2007·The Journal of Thoracic and Cardiovascular Surgery·Martin StrüberJens M Hohlfeld
Apr 14, 2016·Cellular and Molecular Life Sciences : CMLS·Joel M WeinbergM A Venkatachalam
May 4, 2021·Journal of Cellular and Molecular Medicine·Sehoon ParkDong Ki Kim

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