Glycine transporter GLYT1 is essential for glycine-mediated protection of human intestinal epithelial cells against oxidative damage.

The Journal of Physiology
Alison HowardBarry Hugo Hirst

Abstract

Glycine protects mammalian intestine against oxidative damage caused by ischaemia-reperfusion (IR) injury and prevents or reverses experimentally-induced colitis. However the mechanism of protection remains largely unknown. The objectives of the current study were to demonstrate directly glycine-mediated protection of human intestinal epithelial cells and to determine the requirement for glycine uptake by the specific transporter GLYT1. Exogenous glycine protected human intestinal Caco-2 and HCT-8 cells against the oxidative agent tert-butylhydroperoxide and reduced the intracellular concentration of reactive oxygen species, when applied prior to but not concomitant with the oxidative challenge. Glycine given prior to oxidative challenge preserved intracellular glutathione concentration but had no effect on the rate of glycine uptake. Protection was dependent on GLYT1 activity, being blocked by a specific GLYT1 inhibitor, supporting a requirement for intracellular glycine accumulation. Maintained intracellular glutathione content is indicated as a mechanism through which the protective effect may in part be mediated. However expression of the genes encoding GLYT1 and the glutathione synthesising enzymes glutamate-cysteine ligas...Continue Reading

References

Jan 1, 1992·Renal Failure·J M Weinberg
Apr 1, 1991·Kidney International·P SilvaF H Epstein
Dec 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J M WeinbergJ A Davis
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J MårtenssonA Meister
Jan 1, 1986·Transactions of the Royal Society of Tropical Medicine and Hygiene·A A Jackson
Jan 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D C MarshJ H Southard
Sep 1, 1995·Scandinavian Journal of Gastroenterology·M LigumskyI Ginsburg
Jul 1, 1996·The American Journal of Physiology·K IkejimaR G Thurman
Feb 1, 1996·The American Journal of Physiology·Z ZhongR G Thurman
Jan 1, 1997·Pharmacology & Therapeutics·S RajendraP R Schofield
Oct 12, 1999·The American Journal of Physiology·N MerendinoM F Kagnoff
Feb 13, 2001·Gastroenterology·G R ChristieB H Hirst
Aug 9, 2001·Nestlé Nutrition Workshop Series. Clinical & Performance Programme·P J ReedsJ B van Goudoever
Nov 28, 2001·Molecular Pharmacology·B N AtkinsonM A Klitenick
Mar 2, 2002·JPEN. Journal of Parenteral and Enteral Nutrition·Mark A LeeJohn C Hall
Feb 18, 2003·Current Opinion in Clinical Nutrition and Metabolic Care·Zhi ZhongJohn J Lemasters
Apr 30, 2003·Neurochemical Research·Kan ZhangZheng Dong
Sep 2, 2003·Gastroenterology·Isao TsuneNobuhiro Sato
Nov 7, 2003·Journal of Vascular Surgery·Sreedhar KallakuriTheresa Jacob
Feb 12, 2004·Archives of Biochemistry and Biophysics·David M KrzywanskiHenry Jay Forman
Feb 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jung H SuhTory M Hagen
Jun 24, 2004·The American Journal of Clinical Nutrition·Alan A JacksonFarook Jahoor
Apr 23, 2005·Infection and Immunity·Shelley N LuckElizabeth L Hartland
Aug 13, 2005·Acta Anaesthesiologica Scandinavica·R Y GundersenP K Opstad

❮ Previous
Next ❯

Citations

Aug 7, 2012·Archives of Pharmacal Research·Xiao-Lai YangYong-Jie Wu
Oct 31, 2012·The British Journal of Nutrition·Wouter H HendriksGuido Bosch
Nov 1, 2013·Nature Reviews. Drug Discovery·Robert J Harvey, Benjamin K Yee
Jan 20, 2011·The Journal of Biological Chemistry·Esperanza JiménezBeatriz López-Corcuera
Jun 29, 2011·Molecular and Cellular Biology·Alvaro Avivar-ValderasJulio A Aguirre-Ghiso
Jun 2, 2011·Biological & Pharmaceutical Bulletin·Alison Howard, Barry Hugo Hirst
Oct 23, 2013·Canadian Journal of Physiology and Pharmacology·Margarita Díaz-FloresDaniel Hernández-Saavedra
Jan 18, 2018·Expert Opinion on Therapeutic Patents·Christopher L Cioffi
Sep 25, 2014·Biomedit︠s︡inskai︠a︡ khimii︠a︡·V N Nikandrov, T V Balashevich
Mar 25, 2017·Oxidative Medicine and Cellular Longevity·Meerza Abdul RazakSenthilkumar Rajagopal
Jan 17, 2019·Journal, Genetic Engineering & Biotechnology·Solomon A Ndoni, Tebekeme Okoko
Jun 19, 2019·Nutrients·Anaïs AlvesBéatrice Morio
Apr 14, 2016·Cellular and Molecular Life Sciences : CMLS·Joel M WeinbergM A Venkatachalam
May 7, 2019·Frontiers in Endocrinology·Joris H J van SadelhoffAletta D Kraneveld
Apr 3, 2010·The Journal of Physiology·Declan F McCole
Sep 15, 2021·Journal of Cachexia, Sarcopenia and Muscle·Laurence GentonJacques Schrenzel
Sep 19, 2021·Journal of Cachexia, Sarcopenia and Muscle·Laurence GentonJacques Schrenzel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.