Glycoconjugates with Neu5Ac(alpha 2,6)Gal(beta 1,4)GlcNAc sequences: a selective lectin-histochemical property of Kurloff cells in guinea pig thymus

Cell and Tissue Research
N OulhajJ Izard

Abstract

The Kurloff cell (KC), a natural killer lymphocyte, contains a large (10-microns diameter) periodic acid-Schiff (PAS)-positive lysosome-like inclusion body called the Kurloff body (KB), which exhibits strong acid phosphatase activity. The presence of Sambucus nigra agglutinin (SNA)-reactive Neu5Ac(alpha 2,6)-D-Gal/Gal-NAc(beta 1,4)GlcNAc oligosaccharide sequences and the absence of the corresponding Neu5Ac(alpha 2,3) Maackia amurensis agglutinin (MAA)-reactive sequence in the major 35-kDa N-glycosylproteins of the complex or hybrid type extracted from purified KC were established by Western-lectin-blotting of cytosolic extracts from purified KC. Moreover, these SNA-reactive sequences, or at least part of them, were shown to be borne by sialidase-sensitive KC acid isophosphatases. Thymic sections rich in KC, from estrogenized guinea pigs were examined by affino-histochemistry with these sialic acid-reactive lectins. The SNA-reactivity of thymic sections was quasi-exclusively confined to KC clusters, whereas the whole thymic section was negative for MAA. KC were not SNA-reactive following preincubation and incubation with 200 mM lactose. When submitted to enzymatic or mild chemical desialylation processes, the SNA-reactivity of t...Continue Reading

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Citations

Jul 1, 1994·The Histochemical Journal·G LandemoreJ Izard
Jan 28, 2004·Behaviour Research and Therapy·Bunmi O OlatunjiPeter J de Jong
Oct 18, 2005·Journal of Behavior Therapy and Experimental Psychiatry·David F TolinJonathan S Abramowitz
Sep 26, 2002·Behaviour Research and Therapy·Craig N SawchukDavid F Tolin
May 8, 2007·Behaviour Research and Therapy·Brett Deacon, Bunmi O Olatunji
Aug 1, 1997·Microscopy Research and Technique·J Izard
Feb 29, 2008·Journal of Psychoactive Drugs·Timothy P CarmodyJohn A Astin

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