Glycogen synthase kinase 3 promotes multicellular development over unicellular encystation in encysting Dictyostelia

EvoDevo
Yoshinori KawabePauline Schaap

Abstract

Glycogen synthase kinase 3 (GSK3) regulates many cell fate decisions in animal development. In multicellular structures of the group 4 dictyostelid Dictyostelium discoideum, GSK3 promotes spore over stalk-like differentiation. We investigated whether, similar to other sporulation-inducing genes such as cAMP-dependent protein kinase (PKA), this role of GSK3 is derived from an ancestral role in encystation of unicellular amoebas. We deleted GSK3 in Polysphondylium pallidum, a group 2 dictyostelid which has retained encystation as an alternative survival strategy. Loss of GSK3 inhibited cytokinesis of cells in suspension, as also occurs in D. discoideum, but did not affect spore or stalk differentiation in P. pallidum. However, gsk3- amoebas entered into encystation under conditions that in wild type favour aggregation and fruiting body formation. The gsk3- cells were hypersensitive to osmolytes, which are known to promote encystation, and to cyst-inducing factors that are secreted during starvation. GSK3 was not itself regulated by these factors, but inhibited their effects. Our data show that GSK3 has a deeply conserved role in controlling cytokinesis, but not spore differentiation in Dictyostelia. Instead, in P. pallidum, one o...Continue Reading

References

Feb 13, 2001·Molecular Cell·C R Thompson, R R Kay
Jan 23, 2003·Journal of Cell Science·James G WakefieldJeremy M Tavaré
Mar 24, 2004·Current Opinion in Cell Biology·Taro Q P Uyeda, Akira Nagasaki
Sep 3, 2004·Development·Christina SchildeJeffrey G Williams
Oct 28, 2006·Science·Pauline SchaapSandra L Baldauf
May 29, 2007·Cellular and Molecular Life Sciences : CMLS·J E Forde, T C Dale
Apr 1, 2008·Eukaryotic Cell·Masatsune TsujiokaTaro Q P Uyeda
Apr 12, 2008·Developmental Biology·Tamao SaitoRobert R Kay
Jul 22, 2008·Molecular Biology and Evolution·Allyson V RitchiePauline Schaap
Apr 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yoshinori KawabePauline Schaap
Jul 30, 2009·Journal of Cell Science·Takashi WatanabeKozo Kaibuchi
Mar 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Christopher S NeumannRobert R Kay
Jul 22, 2011·Trends in Parasitology·Hugo Aguilar-DíazJorge Morales-Montor
May 18, 2013·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·A Y K Masud RanaShigehiko Yumura
Jun 22, 2013·European Journal of Cell Biology·Adrian J HarwoodAnnette Müller-Taubenberger
Sep 17, 2013·Molecular Reproduction and Development·Amy Bejsovec
Apr 15, 2014·Developmental Biology·William F Loomis
Aug 19, 2015·Journal of Molecular Biology·Qingyou DuPauline Schaap
Jul 1, 2016·Nature Communications·Gernot GlöcknerPauline Schaap

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
environmental stress

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

© 2022 Meta ULC. All rights reserved