Glycogen synthase kinase-3 regulates the phosphorylation of severe acute respiratory syndrome coronavirus nucleocapsid protein and viral replication.

The Journal of Biological Chemistry
Chia-Hsin WuPei-Jer Chen


Coronavirus (CoV) nucleocapsid (N) protein is a highly phosphorylated protein required for viral replication, but whether its phosphorylation and the related kinases are involved in the viral life cycle is unknown. We found the severe acute respiratory syndrome CoV N protein to be an appropriate system to address this issue. Using high resolution PAGE analysis, this protein could be separated into phosphorylated and unphosphorylated isoforms. Mass spectrometric analysis and deletion mapping showed that the major phosphorylation sites were located at the central serine-arginine (SR)-rich motif that contains several glycogen synthase kinase (GSK)-3 substrate consensus sequences. GSK-3-specific inhibitor treatment dephosphorylated the N protein, and this could be recovered by the constitutively active GSK-3 kinase. Immunoprecipitation brought down both N and GSK-3 proteins in the same complex, and the N protein could be phosphorylated directly at its SR-rich motif by GSK-3 using an in vitro kinase assay. Mutation of the two priming sites critical for GSK-3 phosphorylation in the SR-rich motif abolished N protein phosphorylation. Finally, GSK-3 inhibitor was found to reduce N phosphorylation in the severe acute respiratory syndrome...Continue Reading


Apr 1, 1997·Virus Research·R Buckland, T F Wild
Oct 23, 1998·Advances in Experimental Medicine and Biology·S M TaharaD J Manno
Apr 12, 2003·The New England Journal of Medicine·Christian DrostenHans Wilhelm Doerr
Apr 12, 2003·The New England Journal of Medicine·Thomas G KsiazekUNKNOWN SARS Working Group
Nov 19, 2003·Biochemical and Biophysical Research Communications·Runtao HeXuguang Li
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Shiou-Hwei YehUNKNOWN National Taiwan University SARS Research Team
May 19, 2004·Biochemistry·Qiulong HuangEdward T Olejniczak
Jun 15, 2004·Biochemical and Biophysical Research Communications·Tetsuya MizutaniShigeru Morikawa
Oct 28, 2004·Journal of Virology·Fernando AlmazánLuis Enjuanes
May 14, 2005·Journal of Virology·Barbara SchelleVolker Thiel
Jan 30, 2007·Proceedings of the National Academy of Sciences of the United States of America·Chi-Ming ChiuDing-Shinn Chen
Dec 1, 2007·International Journal of Mass Spectrometry·Liang LinSiqi Liu

❮ Previous
Next ❯


Jan 15, 2014·Antiviral Research·Chung-ke ChangTai-huang Huang
Aug 12, 2014·Viruses·Ruth McBrideBurtram C Fielding
Dec 18, 2015·Antiviral Research·Yoshikazu TanakaTakashi Sasaki
Jan 11, 2016·Wiley Interdisciplinary Reviews. RNA·Binbin DingMingzhou Chen
Nov 25, 2011·Reviews in Medical Virology·Julie A Keating, Rob Striker
Apr 2, 2020·Cells·Leonie HoffmeisterRené Huber
May 6, 2020·British Journal of Pharmacology·Hong ZhouXiao-Ming Meng
Jul 4, 2020·Journal of Medical Virology·Satarudra Prakash SinghThakur Prasad Yadav
Jun 21, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sean M Cascarina, Eric D Ross
Feb 20, 2016·Acta Crystallographica. Section D, Structural Biology·Nicolas PapageorgiouBruno Coutard
Jan 16, 2019·Acta Crystallographica. Section D, Structural Biology·Thi Hong Van NguyenBruno Coutard
Dec 14, 2018·Frontiers in Microbiology·Maged SalehChristian M Lange
Aug 20, 2020·Journal of Translational Medicine·Laure Perrin-CoconPierre-Olivier Vidalain
Jun 1, 2018·Future Virology·To Sing Fung, Ding Xiang Liu
Jun 22, 2019·Annual Review of Microbiology·To Sing Fung, Ding Xiang Liu
Jul 23, 2020·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Jatin MachhiBhavesh D Kevadiya
Aug 28, 2020·Frontiers in Microbiology·Katia FecchiAnna Ruggieri
Aug 9, 2020·Computational and Structural Biotechnology Journal·Sailen Barik
Dec 3, 2020·Frontiers in Cellular and Infection Microbiology·Rinki MinakshiJihoe Kim
Feb 3, 2021·Cells·Melinda E SnitowPeter S Klein
Jan 5, 2021·Frontiers in Molecular Biosciences·Giuseppina MarianoJulien R C Bergeron
Feb 12, 2021·Progress in Lipid Research·Ilaria CasariMarco Falasca
Feb 20, 2021·Journal of Proteome Research·Wout BittremieuxNuno Bandeira
Feb 9, 2021·Journal of Molecular Biology·Kristina V TugaevaNikolai N Sluchanko

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.