Abstract
A glycogen synthase, designated GS II, which occurs in a protein/carbohydrate complex has been purified from Ascaris suum muscle. The purified GS-II complex which is eluted from concanavalin-A--Sepharose contains proteins with Mr 140,000 and 66,000 and a glycoprotein with a carbohydrate/protein mass ratio of 3:1. GS II activity was totally dependent on glucose 6-phosphate, but exogenous glycogen was not required for polysaccharide synthesis. The GS-II complex was not phosphorylated by cyclic-AMP-dependent protein kinase, and antibodies to the protein and carbohydrate components of GS II did not cross react with the purified cyclic-AMP-regulated glycogen synthase (GS I) from A. suum muscle. Polysaccharide which was synthesized de novo by the complex was added to the large-molecular-mass glycoprotein in GS II. The glycogen-like character of the newly synthesized polysaccharide was confirmed by the observation that glycogen phosphorylase utilized the polymer as substrate in both the synthesis and degradation reactions. A model is discussed in which a core glycoprotein serves as the substrate for a glycogen synthase which is distinctly different from GS I.
References
Jan 1, 1979·Annual Review of Biochemistry·E G Krebs, J A Beavo
Oct 1, 1978·Archives of Biochemistry and Biophysics·K KawaguchiJ Preiss
May 15, 1975·FEBS Letters·R BarengoC R Krisman
Mar 3, 1975·European Journal of Biochemistry·C R Krisman, R Barengo
May 7, 1976·Analytical Biochemistry·M M Bradford
Dec 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J LomakoW J Whelan
Apr 1, 1986·European Journal of Biochemistry·M L Blumenfeld, C R Krisman
Dec 15, 1987·European Journal of Biochemistry·J PitcherP Cohen
Feb 1, 1988·Archives of Biochemistry and Biophysics·I R Rodriguez, S J Fliesler
Jun 1, 1988·Experimental Parasitology·M E SarcironA F Petavy
Oct 30, 1985·Biochemical and Biophysical Research Communications·I R Rodriguez, W J Whelan
Jul 1, 1969·Analytical Biochemistry·R M ZachariusJ J Woodlock
Aug 15, 1970·Nature·U K Laemmli
Oct 24, 1968·Analytical Biochemistry·J A ThomasJ Larner
Jul 16, 1981·Biochemical and Biophysical Research Communications·M J DonahueB G Harris
Dec 1, 1983·Molecular and Biochemical Parasitology·N J YacoubB G Harris
Sep 1, 1983·European Journal of Biochemistry·M L BlumenfeldC R Krisman
Apr 1, 1981·Molecular and Biochemical Parasitology·B L Allen, B G Harris
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS
Citations
Feb 1, 1997·International Journal for Parasitology·C A Behm
Feb 24, 2001·The Journal of Parasitology·R A MasaracchiaM J Donahue
May 9, 2018·Biochemistry. Biokhimii︠a︡·N N BezborodkinaB N Kudryavtsev
Sep 6, 2001·The Journal of Parasitology·T Z GalH Koltai