PMID: 6181261Aug 1, 1982

Glycol methacrylate embedding of bone and cartilage for light microscopic staining

Journal of Microscopy
M B Cole

Abstract

A method is described for embedding bone and cartilage in glycol methacrylate (GMA) for light microscopy. Dehydration-infiltration of the hard tissue is with aqueous GMA solutions minimizing solvent and dehydration artefact, and polymerization is by UV light in the cold to minimize thermal damage. Over fifty stains, enzyme localizations and related histochemical methods for 0.5-3.0 micrometer thick sections of GMA embedded tissue are listed. The increased resolution plus the localization of cellular and extracellular chemical moieties is now easier and more accurate providing an improved method for the study of the musculo-skeletal system by light microscopy histochemistry.

References

Oct 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P Prentø
Mar 1, 1979·Journal of Microscopy·W J Swartz, F R Nusbickel
Oct 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S CraigA Millerd
Mar 1, 1979·The Histochemical Journal·F R Nusbickel, W J Swartz
Feb 1, 1975·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L L Muller, T J Jacks
Sep 1, 1971·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Hoshino, H Kobayashi
Dec 1, 1972·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A E AshfordM E McCully
Sep 1, 1972·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Hoshino, H Kobayashi
Jul 1, 1967·Journal of Ultrastructure Research·E H Leduc, W Bernhard
Jul 1, 1981·Journal of Microscopy·M B Cole, J Ellinger
Apr 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J A CarnegieH A Robertson

Citations

Dec 4, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jinling FuXiangyun Wei
Dec 28, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·O LabouxA Nanci
Oct 1, 1985·The American Journal of Anatomy·A A Cole, F H Wezeman
Jan 1, 1987·Stain Technology·M Hott, P J Marie
Jan 1, 1991·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·S Lozanoff, J Johnston
Jan 1, 1993·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·S K BulstraA J van der Linden

Related Concepts

Skeletal Bone
Cartilage
Embryonic Structures, Mammalian
Histological Techniques
Electron Microscopy
Polyhydroxyethyl Methacrylate
Staining and Labeling

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