PMID: 6167720Jul 1, 1981Paper

Glycol methacrylate in light microscopy: nucleic acid cytochemistry

Journal of Microscopy
M B Cole, J Ellinger

Abstract

Techniques utilizing Feulgen, azure B bromide, methyl green-pyronin, gallocyanin chromalum and cresyl violet stains have been modified and adapted for visualizing nucleic acids in 0.5-2.0 micrometer sections of tissues embedded in glycol methacrylate (GMA). Methods for evaluating the stain specificity for DNA and RNA using deoxyribonuclease and ribonuclease digestions, aldehyde blocking, and acid extractions are also described. The specificity of the stains in GMA embedded tissues is comparable to that reported for paraffin-embedded tissues.

References

Aug 7, 1953·Science·E H NEWCOMER
Jan 1, 1954·Stain Technology·O ITIKAWA, Y OGURA
Sep 1, 1955·Stain Technology·N B KURNICK
Jun 1, 1964·Journal of Ultrastructure Research·V MARINOZZI
Jan 1, 1961·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C RITTERA FARAH

Related Concepts

Acrylates
Cytological Techniques
DNA, Double-Stranded
Glycols
Intestines, Small
Methacrylates
Ovary
RNA
Staining and Labeling
Testis

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