Glycolate and glyoxylate metabolism in HepG2 cells

American Journal of Physiology. Cell Physiology
Paul R S BakerRoss P Holmes

Abstract

Oxalate synthesis in human hepatocytes is not well defined despite the clinical significance of its overproduction in diseases such as the primary hyperoxalurias. To further define these steps, the metabolism to oxalate of the oxalate precursors glycolate and glyoxylate and the possible pathways involved were examined in HepG2 cells. These cells were found to contain oxalate, glyoxylate, and glycolate as intracellular metabolites and to excrete oxalate and glycolate into the medium. Glycolate was taken up more effectively by cells than glyoxylate, but glyoxylate was more efficiently converted to oxalate. Oxalate was formed from exogenous glycolate only when cells were exposed to high concentrations. Peroxisomes in HepG2 cells, in contrast to those in human hepatocytes, were not involved in glycolate metabolism. Incubations with purified lactate dehydrogenase suggested that this enzyme was responsible for the metabolism of glycolate to oxalate in HepG2 cells. The formation of 14C-labeled glycine from 14C-labeled glycolate was observed only when cell membranes were permeabilized with Triton X-100. These results imply that peroxisome permeability to glycolate is restricted in these cells. Mitochondria, which produce glyoxylate fro...Continue Reading

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Citations

Sep 16, 2010·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·J KnightR P Holmes
Aug 16, 2013·The New England Journal of Medicine·Pierre Cochat, Gill Rumsby
Nov 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Eunhye GooIngyu Hwang
Mar 3, 2010·Journal of Experimental Botany·Le YuXinxiang Peng
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Dec 31, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Juquan JiangW Todd Lowther
Apr 26, 2005·American Journal of Nephrology·John Knight, Ross P Holmes
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Dec 20, 2014·Journal of Diabetes Research·Victoria J NikiforovaDietrich Rein
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Feb 3, 2021·Communications Biology·Veronica DiezTeymuras Vakhtang Kurzchalia

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