Glycolic Acid Silences Inflammasome Complex Genes, NLRC4 and ASC, by Inducing DNA Methylation in HaCaT Cells

DNA and Cell Biology
Sheau-Chung TangJen-Hung Yang

Abstract

AHAs (α-hydroxy acids), including glycolic acid (GA), have been widely used in cosmetic products and superficial chemical peels. Inflammasome complex has been shown to play critical roles in inflammatory pathways in human keratinocytes. However, the anti-inflammatory mechanism of GA is still unknown. The aim of this study is to investigate the relationship between the expression of the inflammasome complex and epigenetic modification to elucidate the molecular mechanism of the anti-inflammatory effect of GA in HaCaT cells. We evaluated NLRP3, NLRC4, AIM2, and ASC inflammasome complex gene expression on real-time polymerase chain reaction (PCR). Methylation changes were detected in these genes following treatment with DNA methyltransferase (DNMT) inhibitor 5-aza-2'-deoxycytidine (5-Aza) with or without the addition of GA using methylation-specific PCR (MSP). GA inhibited the expressions of these inflammasome complex genes, and the decreases in the expressions of mRNA were reversed by 5-Aza treatment. Methylation was detected in NLRC4 and ASC on MSP, but not in NLRP3 or AIM2. GA decreased NLRC4 and ASC gene expression by increasing not only DNA methyltransferase 3B (DNMT-3B) protein level, but also total DNMT activity. Furthermor...Continue Reading

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Citations

Sep 24, 2019·Journal of Cosmetic Dermatology·Amira Amin ZayedSara Bahaa Mahmoud
Apr 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sheau-Chung Tang, Jen-Hung Yang
Jul 25, 2021·International Journal of Molecular Sciences·Young-Su Yi

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Methods Mentioned

BETA
PCR
electrophoresis
Cytomation
Assay
histone acetylation

Software Mentioned

ImageJ
MethPrimer

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