Glycolipid composition of the heterocyst envelope of Anabaena sp. PCC 7120 is crucial for diazotrophic growth and relies on the UDP-galactose 4-epimerase HgdA

MicrobiologyOpen
Dmitry ShvarevIris Maldener

Abstract

The nitrogenase complex in the heterocysts of the filamentous freshwater cyanobacterium Anabaenasp. PCC 7120 fixes atmospheric nitrogen to allow diazotrophic growth. The heterocyst cell envelope protects the nitrogenase from oxygen and consists of a polysaccharide and a glycolipid layer that are formed by a complex process involving the recruitment of different proteins. Here, we studied the function of the putative nucleoside-diphosphate-sugar epimerase HgdA, which along with HgdB and HgdC is essential for deposition of the glycolipid layer and growth without a combined nitrogen source. Using site-directed mutagenesis and single homologous recombination approach, we performed a thoroughly functional characterization of HgdA and confirmed that the glycolipid layer of the hgdAmutant heterocyst is aberrant as shown by transmission electron microscopy and chemical analysis. The hgdA gene was expressed during late stages of the heterocyst differentiation. GFP-tagged HgdA protein localized inside the heterocysts. The purified HgdA protein had UDP-galactose 4-epimerase activity in vitro. This enzyme could be responsible for synthesis of heterocyst-specific glycolipid precursors, which could be transported over the cell wall by the AB...Continue Reading

References

Jan 1, 1988·Methods in Enzymology·J Elhai, C P Wolk
Jul 19, 1974·Nature·M G RossmannK W Olsen
Jan 1, 1972·Archiv für Mikrobiologie·P Fay, S A Kulasooriya
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C P WolkJ Elhai
Sep 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C R Bellamacina
May 10, 2000·Nature Structural Biology·M F GiraudJ H Naismith
Nov 15, 2001·Cellular and Molecular Life Sciences : CMLS·S T AllardJ H Naismith
Oct 1, 1953·Journal of Bacteriology·R E McKINNEY
Dec 22, 2005·Nature Biotechnology·Jean-Denis PédelacqGeoffrey S Waldo
Jun 8, 2007·Biochimica Et Biophysica Acta·Shivani AgarwalAparna Dixit
Jul 24, 2007·Trends in Microbiology·Anthony E Walsby
Jul 28, 2007·Biological Chemistry·Suncana MoslavacEnrico Schleiff
Apr 22, 2008·Nucleic Acids Research·A DereeperO Gascuel
Mar 3, 2009·Journal of Basic Microbiology·Kerstin NicolaisenEnrico Schleiff
Apr 14, 2009·Nature Methods·Daniel G GibsonHamilton O Smith
Sep 24, 2009·Phytochemistry·Thorsten BauersachsJaap S Sinninghe Damsté
Dec 8, 2009·Nature Reviews. Microbiology·Enrique Flores, Antonia Herrero
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Krithika KumarJames W Golden
Sep 16, 2011·The Journal of Biological Chemistry·Peter StaronIris Maldener
Aug 18, 2012·Trends in Microbiology·Alicia M Muro-Pastor, Wolfgang R Hess
Dec 20, 2012·Applied Microbiology and Biotechnology·Koen BeerensTom Desmet
Sep 21, 2013·Biochimica Et Biophysica Acta·Laurence BoudièreEric Maréchal
Jul 15, 2015·Carbohydrate Research·Koen BeerensTom Desmet

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Citations

Feb 20, 2020·The Journal of General and Applied Microbiology·Tsukasa Saito, Koichiro Awai

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Methods Mentioned

BETA
PCR
electrophoresis
fluorescence microscopy
light microscopy
transmission electron microscopy

Software Mentioned

Swiss model
Leica ASF
Phylogeny
BLAST
GelQuantNET
PhyML
Swiss
PaperBLAST
fr

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