PMID: 2497776Mar 7, 1989Paper

Glycolipid glycosyltransferases in human embryonal carcinoma cells during retinoic acid induced differentiation

Biochemistry
C ChenS Hakomori

Abstract

Retinoic acid induced differentiation of TERA-2-derived human embryonal carcinoma cells is accompanied by a dramatic reduction of extended globo-series glycolipids, including galactosyl globoside, sialylgalactosyl globoside, and globo-A antigen (each recognized by specific MoAbs). Associated with these glycolipid changes, the activities of two key enzymes, alpha 1----4 galactosyltransferase (for synthesis of globotriaosyl core structure) and beta 1----3 galactosyltransferase (for synthesis of galactosyl globoside), were found to be reduced 3- to 4-fold. The latter enzyme plays a key role in the synthesis of extended globo-series structures, and its characterization has not been reported previously. Therefore, its catalytic activity was studied in detail, including substrate specificity, detergent and phospholipid effects, pH and cation requirements, and apparent Km. During retinoic acid induced differentiation, a series of Lex glycolipid antigens (recognized by anti-SSEA-1 antibody) and their core structures (lacto-series type 2 chains) increase dramatically. In parallel with these changes in glycolipid expression, the activities of two key enzymes, beta 1----3 N-acetylglucosaminyltransferase (for extension of lacto-series type...Continue Reading

References

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Citations

Apr 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·B A FendersonS Hakomori
Mar 31, 1994·European Journal of Obstetrics, Gynecology, and Reproductive Biology·L VitaioliS R Indraccolo
Feb 17, 2000·Brain Research. Developmental Brain Research·B NicoL Roncali
Sep 12, 2012·The Journal of Biological Chemistry·Anna SuchanowskaMarcin Czerwinski
Feb 14, 2013·The Journal of Biological Chemistry·Angela BaroneMichael E Breimer
Jul 1, 1994·International Journal of Cancer. Journal International Du Cancer·J WenkB A Fenderson
Nov 15, 1989·Biochemical and Biophysical Research Communications·M P SkinnerM C Berndt
Apr 29, 2009·Stem Cell Research·Andrew J Wright, Peter W Andrews
Jun 20, 2016·Glycoconjugate Journal·Kazuko Handa, Sen-Itiroh Hakomori
Dec 1, 1992·Journal of Neurochemistry·R Li, S Ladisch
Jun 1, 1992·The American Journal of Physiology·C B Zeller, R B Marchase
Jul 1, 1989·Trends in Biochemical Sciences·J C Paulson

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