Glycolysis is essential for chemoresistance induced by transient receptor potential channel C5 in colorectal cancer

BMC Cancer
Teng WangDong Hua

Abstract

Elevated intracellular Ca2+ ([Ca2+] i ) level could lead to [Ca2+] i overload and promote apoptosis via different pathways. In our previously study, up-regulated expression of transient receptor potential canonical channel (TRPC5) was proven to increase [Ca2+] i level, and resulted in chemoresistance whereas not apoptosis in human colorectal cancer (CRC) cells. The ATP-dependent homeostatic maintenance of resting [Ca2+] i should be important in this process. Increased glycolysis was found to be an important adenosine triphosphate (ATP) source in cancer. This study aimed to explore the potential mechanism of aerobic glycolysis in transient receptor potential channel TRPC5 induced chemoresistance. In this study, we examined glucose transporter 1 (GLUT1) expression, glucose consumption and celluar ATP production to determine glycolytic activity. Real-time PCR and western blot were analyzed to determine TRPC5 expression at the mRNA and protein levels in human CRC cells (HCT-8, LoVo), and fluorouracil (5-Fu) resistant CRC cells (HCT-8/5-Fu, LoVo/5-Fu). 3-bromopyruvate (3-BP) and 2-Deoxy-D-glucose (2DG) were used to inhibit glycolysis. Glycolytic activity, intracellular Ca2+ ([Ca2+] i ) and the half maximal inhibitory concentration o...Continue Reading

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Mar 20, 2019·Cancers·Philippe KischelHalima Ouadid-Ahidouch
Dec 12, 2019·Frontiers in Oncology·Chung-Yen HuangLinda Chia-Hui Yu
Jun 23, 2019·International Journal of Molecular Sciences·Elizabeth VargheseDietrich Büsselberg
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Jan 9, 2021·Neoplasia : an International Journal for Oncology Research·Fabrizio Marcucci, Cristiano Rumio
Nov 19, 2021·Drug Discovery Today·Suman PandaSubhrangsu Chatterjee

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BETA
PCR
biopsy

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