Glycome profiling by lectin microarray reveals dynamic glycan alterations during epidermal stem cell aging.

Aging Cell
Lalhaba OinamHiromi Yanagisawa

Abstract

Aging in the epidermis is marked by a gradual decline in barrier function, impaired wound healing, hair loss, and an increased risk of cancer. This could be due to age-related changes in the properties of epidermal stem cells and defective interactions with their microenvironment. Currently, no biochemical tools are available to detect and evaluate the aging of epidermal stem cells. The cellular glycosylation is involved in cell-cell communications and cell-matrix adhesions in various physiological and pathological conditions. Here, we explored the changes of glycans in epidermal stem cells as a potential biomarker of aging. Using lectin microarray, we performed a comprehensive glycan profiling of freshly isolated epidermal stem cells from young and old mouse skin. Epidermal stem cells exhibited a significant difference in glycan profiles between young and old mice. In particular, the binding of a mannose-binder rHeltuba was decreased in old epidermal stem cells, whereas that of an α2-3Sia-binder rGal8N increased. These glycan changes were accompanied by upregulation of sialyltransferase, St3gal2 and St6gal1 and mannosidase Man1a genes in old epidermal stem cells. The modification of cell surface glycans by overexpressing these...Continue Reading

References

Jul 1, 1983·The Journal of Investigative Dermatology·B A Gilchrest
Feb 14, 2002·The Journal of Investigative Dermatology·Andreas WollenbergMartina Moderer
Jun 11, 2004·Annual Review of Biochemistry·Robert S Haltiwanger, John B Lowe
Oct 7, 2004·Glycoconjugate Journal·Jun Hirabayashi
Oct 7, 2004·Glycoconjugate Journal·Takashi Muramatsu, Hisako Muramatsu
Sep 9, 2006·Cell·Kazuaki Ohtsubo, Jamey D Marth
Jun 19, 2007·Nature Biotechnology·UNKNOWN International Stem Cell InitiativeWeidong Zhang
Aug 19, 2007·Glycobiology·Hiroaki TatenoJun Hirabayashi
Dec 7, 2007·Annals of the New York Academy of Sciences·E Makrantonaki, C C Zouboulis
Jan 29, 2008·Aging Cell·Adam GiangrecoFiona M Watt
May 12, 2009·Glycobiology·Ajit Varki
May 22, 2009·The Journal of Investigative Dermatology·Alexandra CharruyerRuby Ghadially
Sep 25, 2009·The Journal of Investigative Dermatology·Adam GiangrecoFiona M Watt
May 28, 2010·Disease Models & Mechanisms·David Piccin, Cindi M Morshead
Mar 29, 2011·Cold Spring Harbor Perspectives in Biology·Fiona M Watt, Hironobu Fujiwara
Apr 8, 2011·The Journal of Biological Chemistry·Hiroaki TatenoMakoto Asashima
Oct 2, 2012·Molecular & Cellular Proteomics : MCP·Kayo HasehiraJun Hirabayashi
Feb 28, 2013·Chemical Society Reviews·Jun HirabayashiHiroaki Tateno
Jun 12, 2013·Cell·Carlos López-OtínGuido Kroemer
Dec 19, 2013·PloS One·Frank HanischRüdiger Horstkorte
Jun 6, 2015·Cell Stem Cell·Peter D AdamsK Lenhard Rudolph
Feb 4, 2016·Oncotarget·Mariangela CateraFabio Dall'Olio
Mar 26, 2016·Mechanisms of Ageing and Development·Abigail K LangtonRachel Eb Watson
Apr 20, 2017·Stem Cell Research & Therapy·Norihiko SasakiMasashi Toyoda
Nov 22, 2017·Developmental Cell·Kevin Andrew Uy Gonzales, Elaine Fuchs
Mar 28, 2018·International Journal of Molecular Sciences·Elina ChermnykhEkaterina Vorotelyak
May 29, 2018·Trends in Cell Biology·Emanuel Rognoni, Fiona M Watt
Apr 5, 2019·Nature·Nan LiuEmi K Nishimura
Feb 26, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yejing GeElaine Fuchs

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation
flow cytometry
PCR
scraping
FACS
Chips

Software Mentioned

FlowJo
TreeView
SPSS
GraphPad Prism8
Java
Cluster
ImageJ

Related Concepts

Related Feeds

Cell Aging (Keystone)

This feed focuses on cellular aging with emphasis on the mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.