Glycomic alterations are associated with multidrug resistance in human leukemia

The International Journal of Biochemistry & Cell Biology
Zhaohai ZhangLi Jia

Abstract

Correlations of disease phenotypes with glycosylation changes have been analyzed intensively in tumor biology field. In this study we describe glycomic alterations of multidrug resistance in human leukemia cell lines. Using multiple glycan profiling tools: real-time PCR for quantification of glycogenes, FITC-lectin binding for glycan profiling, and mass spectrometry for glycan composition, we compared the glycomics of drug-resistant K562/ADR cells with parental K562 line. The results showed that the expression of glycogenes, glycan profiling and N-glycan composition were different in K562/ADR cells, as compared with those in K562 cells, whereas O-glycans of the two cell lines showed no different mass spectra. Further analysis of the N-glycan regulation by way of tunicamycin application or PNGase F treatment in K562/ADR cells showed partial inhibition of biosynthesis and increased sensitivity to chemotherapeutic drugs in vitro. We targeted glycogene B3GNT8 and ST8SIA4, which were over-expressed in K562/ADR cells, and silenced the expression levels of two glycogenes after using RNA interference approach. The results showed that the silencing of B3GNT8 or ST8SIA4 in K562/ADR cells resulted in increased chemosensitivity to anti-tum...Continue Reading

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Citations

Apr 18, 2014·International Journal of Molecular Sciences·Yang BaiZhi-Nan Chen
Jun 21, 2014·Biochimica Et Biophysica Acta·Fabio Dall'OlioMariella Chiricolo
Sep 29, 2015·Expert Review of Anticancer Therapy·Fei FeiZhinan Chen
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Apr 19, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yan LiLi Jia
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Mar 31, 2021·Drug Discovery Today·Mehrdad IzadiradZhe-Sheng Chen
Jul 27, 2021·Experimental and Therapeutic Medicine·Yanbin SongChen Chen

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