Smooth muscle cells from rat heart secreted extracellular matrix components at high rates for many generations in culture. The matrix proteins remained anchored to the culture dish and were characterized after removal of cellular material with sodium dodecyl sulfate. Sequential enzyme digestion demonstrated the presence of at least three components, including glycoprotein(s), elastin, and collagen. Prolonged extraction of the matrix with detergent under reducing conditions solubilized a fucosylated glycoprotein having an apparent molecular weight of 250,000 and two other proteins with molecular weights of 72,000 and 45,000, respectively. Sublines derived from discrete colonies of smooth muscle cells synthesized all of the matrix components, and the proportion of collagen secreted by some sublines increased with time in culture. The biosynthesis of a mixed extracellular matrix and the relationships among the component proteins were therefore studied in one system producing milligram quantities of material.
Ascorbate-independent proline hydroxylation resulting from viral transformation of Balb 3T3 cells and unaffected by dibutyryl cAMP treatment
Dependence of the differentiated state on the cellular environment: modulation of collagen synthesis in tendon cells
Characterization of the collagen chains synthesized by cultured smooth muscle cells derived from rhesus monkey thoracic aorta
Synthesis of elastin in aortas from chick embryos. Conversion of newly secreted elastin to cross-linked elastin without apparent proteolysis of the molecule
A presumptive subunit of elastic fiber microfibrils secreted by arterial smooth-muscle cells in culture
Improved resolution of myofibrillar proteins with sodium dodecyl sulfate-polyacrylamide gel electrophoresis
Increased collagen synthesis in Kirsten sarcoma virus-transformed BALB 3T3 cells grown in the presence of dibutyryl cyclic AMP
Modulation of extracellular-matrix synthesized by cultured stromal cells from normal human breast tissue by epidermal growth factor
Sertoli cells in culture secrete paracrine factor(s) that inhibit peritubular myoid cell proliferation: identification of heparinoids as likely candidates
Sphingosine reverses growth inhibition caused by activation of protein kinase C in vascular smooth muscle cells
Histological differentiation in human breast cancer is related to steroid receptors and stromal elastosis
Three-dimensional organization of the extracellular matrix secreted by cultured rat smooth muscle cells
An ultrastructural study on ventricular encapsulation reactions in Biomphalaria glabrata exposed to irradiated echinostome parasites
Ascorbate increases the number of low density lipoprotein receptors in cultured arterial smooth muscle cells
Establishment of a rhabdoid tumor cell line with a specific chromosomal abnormality, 46,XY,t(11;22)(p15.5;q11.23)
The effect of ascorbate on the synthesis of minor (non-interstitial) collagens by cultured bovine aortic smooth muscle cells
The role of plasma semicarbazide-sensitive amine oxidase in allylamine and beta-aminopropionitrile cardiovascular toxicity: mechanisms of myocardial protection and aortic medial injury in rats
Mouse L cells expressing human prourokinase-type plasminogen activator: effects on extracellular matrix degradation and invasion
Metastatic behavior of human melanoma cell lines in nude mice correlates with urokinase-type plasminogen activator, its type-1 inhibitor, and urokinase-mediated matrix degradation
Degradation of connective tissue matrices by macrophages. I. Proteolysis of elastin, glycoproteins, and collagen by proteinases isolated from macrophages
Human and rat mesangial cell receptors for glucose-modified proteins: potential role in kidney tissue remodelling and diabetic nephropathy
Cathepsin-D-dependent initiation of the hydrolysis by lysosomal enzymes of apoprotein B from low-density lipoproteins
Atrial natriuretic peptide induces breakdown of phosphatidylinositol phosphates in cultured vascular smooth-muscle cells
Activation of multiple signal transduction pathways by endothelin in cultured human vascular smooth muscle cells
Endothelin-induced increases in vascular smooth muscle Ca2+ do not depend on dihydropyridine-sensitive Ca2+ channels
Receptor-specific induction of insulin-like growth factor I in human monocytes by advanced glycosylation end product-modified proteins
Mechanical strain and collagen potentiate mitogenic activity of angiotensin II in rat vascular smooth muscle cells
Mechanical strain of rat vascular smooth muscle cells is sensed by specific extracellular matrix/integrin interactions
Increased sulphation level and altered composition of glycosaminoglycans synthesized by cultured smooth muscle cells in the presence of beta-D-xylosides
Decreased susceptibility of cultured smooth muscle cells from SHR rats to growth inhibition by heparin
Extracellular matrix: differential influence on growth and biosynthesis patterns of vascular smooth muscle cells from SHR and WKY rats
Verapamil stereoisomers induce antiproliferative effects in vascular smooth muscle cells via autophagy
A role for the cell cycle phosphatase Cdc25a in vitamin D-dependent inhibition of adult rat vascular smooth muscle cell proliferation
Vitamin D-dependent suppression of endothelin-induced vascular smooth muscle cell proliferation through inhibition of CDK2 activity
20-Hydroxyeicosatetraenoic acid inhibits ATP-induced COX-2 expression via peroxisome proliferator activator receptor-α in vascular smooth muscle cells
The mast cell as an effector of connective tissue degradation: a study of matrix susceptibility to human mast cells
Three-dimensional fine structure of elastic fibers in the perivascular space of some circumventricular organs as revealed by high-voltage electron microscopy
Dissociation between activation of growth-related genes and mitogenic responses of neonatal vascular smooth muscle cells
The elastogenic effect of recombinant transforming growth factor-beta on porcine aortic smooth muscle cells
Differential stimulation of growth related metabolism in cultured smooth muscle cells from SHR and WKY rats by combinations of EGF and LDL
Activation of S6 kinase in cultured vascular smooth muscle cells by submitogenic levels of thrombospondin
An essential requirement for β1 integrin in the assembly of extracellular matrix proteins within the vascular wall
TGF-beta1-induced plasminogen activator inhibitor-1 expression in vascular smooth muscle cells requires pp60(c-src)/EGFR(Y845) and Rho/ROCK signaling
Role of glutathione S-transferase 8-8 in allylamine resistance of vascular smooth muscle cells in vitro
The role of glutathione S-transferases as a defense against reactive electrophiles in the blood vessel wall
Platelet-derived growth factor AA homodimer stimulates protein synthesis rather than DNA synthesis in vascular smooth muscle cells from spontaneously hypertensive rats but not from normotensive rats
20-HETE inhibits the proliferation of vascular smooth muscle cells via transforming growth factor-beta.
Activation of matrix metalloproteinase-9 (MMP-9) via a converging plasmin/stromelysin-1 cascade enhances tumor cell invasion.
Cultured rat aortic vascular smooth muscle cells digest naturally produced extracellular matrix. Involvement of plasminogen-dependent and plasminogen-independent pathways
Epidermal growth factor responsiveness in smooth muscle cells from hypertensive and normotensive rats
Enhanced responsiveness to angiotensin II in vascular smooth muscle cells from spontaneously hypertensive rats is not associated with alterations in protein kinase C
Rapid induction and translocation of Egr-1 in response to mechanical strain in vascular smooth muscle cells
Myocyte hypertrophy in neonatal rat heart cultures and its regulation by serum and by catecholamines
Comparison of plasminogen binding and activation on extracellular matrices produced by vascular smooth muscle and endothelial cells
Visualization of the three-dimensional distribution of collagen fibrils over preovulatory follicles in the hamster
Culture of human cells and synthesis of extracellular matrix on materials compatible with direct analysis by mass spectrometry
B-Myb expression in vascular smooth muscle cells occurs in a cell cycle-dependent fashion and down-regulates promoter activity of type I collagen genes.
Interaction of matrix metalloproteinases with serine protease inhibitors. New potential roles for matrix metalloproteinase inhibitors
Glycosaminoglycan synthesis by smooth muscle cells cultured in the absence and presence of ascorbic acid
Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.
Neural Activity: Imaging
Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.
The Tendon Seed Network
Tendons are rich in the extracellular matrix and are abundant throughout the body providing essential roles including structure and mobility. The transcriptome of tendons is being compiled to understand the micro-anatomical functioning of tendons. Discover the latest research pertaining to the Tendon Seed Network here.
Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.
Chronic Fatigue Syndrome
Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.
Incretins are metabolic hormones that stimulate a decrease in glucose levels in the blood and they have been implicated in glycemic regulation in the remission phase of type 1 diabetes. Here is the latest research.
Chromatin Regulation and Circadian Clocks
The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.
“Long Covid-19” describes illness in patients who are reporting long-lasting effects of the SARS-CoV-19 infection, often long after they have recovered from acute Covid-19. Ongoing health issues often reported include low exercise tolerance and breathing difficulties, chronic tiredness, and mental health problems such as post-traumatic stress disorder and depression. This feed follows the latest research into Long Covid.
Spatio-Temporal Regulation of DNA Repair
DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.