Glycoprotein nmb Is Exposed on the Surface of Dormant Breast Cancer Cells and Induces Stem Cell-like Properties

Cancer Research
Chen ChenMitsuyasu Kato

Abstract

Glycoprotein nmb (GPNMB) is a type I transmembrane protein that contributes to the initiation and malignant progression of breast cancer through induction of epithelial-mesenchymal transition (EMT). Although it is known that EMT is associated with not only cancer invasion but also acquisition of cancer stem cell (CSC) properties, the function of GPNMB in this acquisition of CSC properties has yet to be elucidated. To address this issue, we utilized a three-dimensional (3D) sphere culture method to examine the correlation between GPNMB and CSC properties in breast cancer cells. Three-dimensional sphere cultures induced higher expression of CSC genes and EMT-inducing transcription factor (EMT-TF) genes than the 2D monolayer cultures. Three-dimensional culture also induced cell surface expression of GPNMB on limited numbers of cells in the spheres, whereas the 2D cultures did not. Therefore, we isolated cell surface-GPNMBhigh and -GPNMBlow cells from the spheres. Cell surface-GPNMBhigh cells expressed high levels of CSC genes and EMT-TF genes, had significantly higher sphere-forming frequencies than the cell surface-GPNMBlow cells, and showed no detectable levels of proliferation marker genes. Similar results were obtained from tr...Continue Reading

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Citations

Nov 13, 2018·Journal of Cellular Physiology·Masoud NajafiKeywan Mortezaee
May 24, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pei-Suen Tsou, Amr H Sawalha
Feb 8, 2020·Analytical and Bioanalytical Chemistry·Yue WangZhixin Tian
Aug 24, 2021·Cancer Science·Daisuke FukushiKeiichi Tamai
Sep 30, 2021·Molecular Medicine·Paola AllavenaCristina Belgiovine

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