Glycoprotein VI but not alpha2beta1 integrin is essential for platelet interaction with collagen

The EMBO Journal
B NieswandtR Fässler

Abstract

Platelet adhesion on and activation by components of the extracellular matrix are crucial to arrest post-traumatic bleeding, but can also harm tissue by occluding diseased vessels. Integrin alpha2beta1 is thought to be essential for platelet adhesion to subendothelial collagens, facilitating subsequent interactions with the activating platelet collagen receptor, glycoprotein VI (GPVI). Here we show that Cre/loxP-mediated loss of beta1 integrin on platelets has no significant effect on the bleeding time in mice. Aggregation of beta1-null platelets to native fibrillar collagen is delayed, but not reduced, whereas aggregation to enzymatically digested soluble collagen is abolished. Furthermore, beta1-null platelets adhere to fibrillar, but not soluble collagen under static as well as low (150 s(-1)) and high (1000 s(-1)) shear flow conditions, probably through binding of alphaIIbbeta3 to von Willebrand factor. On the other hand, we show that platelets lacking GPVI can not activate integrins and consequently fail to adhere to and aggregate on fibrillar as well as soluble collagen. These data show that GPVI plays the central role in platelet-collagen interactions by activating different adhesive receptors, including alpha2beta1 inte...Continue Reading

References

Sep 11, 1975·The New England Journal of Medicine·H J Weiss
May 3, 1991·Cell·D R PhillipsR M Scarborough
Sep 8, 1995·Science·R KühnK Rajewsky
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M LenterD Vestweber
Oct 1, 1996·Current Opinion in Cell Biology·R FässlerE Hirsch
Jun 26, 1998·Immunology Today·S P Watson, J Gibbins
Oct 17, 1998·Current Opinion in Hematology·M J BarnesR W Farndale
Mar 21, 2000·Seminars in Immunology·P W JanesP S Kabouridis
Aug 30, 2000·Biochemical Society Transactions·M J Humphries
Oct 19, 2000·The Journal of Biological Chemistry·V SchulteB Nieswandt
Feb 22, 2001·The Journal of Experimental Medicine·B NieswandtH Zirngibl

❮ Previous
Next ❯

Citations

Oct 17, 2002·The Anatomical Record·Jean-Yves ExpositoClaire Lethias
Nov 9, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·David Stegner, Bernhard Nieswandt
Oct 6, 2005·Histochemistry and Cell Biology·Pascal MauriceFrançoise Fauvel-Lafève
Jun 12, 2008·Pflügers Archiv : European journal of physiology·David Varga-SzaboBernhard Nieswandt
Aug 16, 2008·Pflügers Archiv : European journal of physiology·Irina PleinesBernhard Nieswandt
Nov 1, 2005·Cancer Metastasis Reviews·C Brakebusch, R Fässler
Nov 20, 2003·The Journal of Laboratory and Clinical Medicine·Makoto KanekoYukio Ozaki
Jan 5, 2011·Thrombosis Research·Benedicte P NuyttensKatleen Broos
Jan 24, 2002·Trends in Pharmacological Sciences·Paolo Gresele, Giancarlo Agnelli
Oct 6, 2007·Journal of Medicinal Chemistry·Sungwook ChoiWilliam F DeGrado
Mar 10, 2009·Journal of Proteome Research·David L SpringerRichard P Phipps
Nov 9, 2011·Nature Medicine·Shaun P Jackson
Aug 31, 2004·Nature Medicine·Jill R CrittendenAnn M Graybiel
Feb 19, 2008·Nature Medicine·Markus MoserReinhard Fässler
Feb 22, 2002·European Journal of Biochemistry·Alexis AquilinaDanny Tuckwell
Aug 30, 2008·The New England Journal of Medicine·Bruce Furie, Barbara C Furie
Oct 10, 2009·The Journal of Biological Chemistry·Robin J MarjoramSamuel A Santoro
May 19, 2010·The Journal of Biological Chemistry·Karen GilioAlastair W Poole
Dec 10, 2008·The Journal of Biological Chemistry·Haihong ZongJeffrey E Pessin
Jan 8, 2003·The Journal of Experimental Medicine·Steffen MassbergBernhard Nieswandt
Dec 19, 2007·The Journal of Experimental Medicine·Brian G PetrichMark H Ginsberg
Dec 19, 2007·The Journal of Experimental Medicine·Bernhard NieswandtReinhard Fässler
Jun 19, 2008·The Journal of Experimental Medicine·David Varga-SzaboBernhard Nieswandt
Mar 6, 2009·American Journal of Therapeutics·Inna ShalitoVictor Serebruany
Sep 23, 2011·Cold Spring Harbor Perspectives in Biology·Wolfgang Bergmeier, Richard O Hynes
Dec 25, 2003·Transfusion·Joyce CurversJohan W M Heemskerk
Dec 17, 2008·Molecular and Cellular Biology·Jenny SchröderPaul Saftig
Jul 2, 2003·Molecular and Cellular Biology·Hong Chen, Mark L Kahn
Dec 3, 2005·The Journal of Clinical Investigation·Meinrad GawazAndreas E May
Oct 30, 2007·The Journal of Clinical Investigation·Johannes GrosseBernhard Nieswandt
Jul 19, 2013·The Journal of Clinical Investigation·Carsten DeppermannBernhard Nieswandt
Dec 2, 2010·Toxicologic Pathology·Andrew J Gale
Jun 2, 2011·Journal of Child Neurology·M Patricia Massicotte, M E Bauman
Apr 18, 2009·Blood·Paola E J van der MeijdenJohan W M Heemskerk
Jul 25, 2009·Blood·Lucia StefaniniWolfgang Bergmeier
Jul 25, 2009·Blood·Alec A Schmaier, Alvin H Schmaier

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.