PMID: 9420258Jan 7, 1998Paper

Glycoproteins M and N of pseudorabies virus form a disulfide-linked complex

Journal of Virology
A JönsThomas C Mettenleiter

Abstract

Genes homologous to the herpes simplex virus UL49.5 open reading frame are conserved throughout the Herpesviridae. In the alphaherpesvirus pseudorabies virus (PrV), the UL49.5 product is an O-glycosylated structural protein of the viral envelope, glycoprotein N (gN) (A. Jöns, H. Granzow, R. Kuchling, and T. C. Mettenleiter, J. Virol. 70:1237-1241, 1996). For functional characterization of gN, a gN-negative PrV mutant, PrV-gNbeta, and the corresponding rescuant, PrV-gNbetaR, were constructed, gN-negative PrV was able to productively replicate on noncomplementing cells, and one-step growth in cell culture was only slightly reduced compared to that of wild-type PrV. However, penetration was significantly delayed. In indirect immunofluorescence assays with rabbit serum directed against baculovirus-expressed gN, specific staining of wild-type PrV-infected cells occurred only after permeabilization of cells, whereas live cells failed to react with the antiserum. This indicates the lack of surface accessibility of gN in the plasma membrane of a PrV-infected cell. Western blot analyses and radioimmunoprecipitation experiments under reducing and nonreducing conditions led to the discovery of a heteromeric complex composed of gM and gN. ...Continue Reading

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Citations

Apr 12, 2001·The Journal of General Virology·B F KinghamC J Schmidt
Nov 24, 2004·The Journal of General Virology·Colin M CrumpHelena M Browne
Sep 9, 2005·Microbiology and Molecular Biology Reviews : MMBR·Lisa E PomeranzChristoph J Hengartner
Aug 27, 2015·Virology Journal·Robyn N HallTimothy J Mahony
Nov 9, 2000·Journal of Virology·C M Lake, L M Hutt-Fletcher
Jul 11, 2000·Journal of Virology·B G KluppT C Mettenleiter
Mar 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Danijela Koppers-LalicEmmanuel J H J Wiertz
Jan 29, 2010·Journal of Virology·Tomohiko SadaokaYasuko Mori
Feb 26, 2016·Viruses·Baptiste LeroyRuddy Wattiez
Feb 26, 2005·Journal of Virology·Janet S MayPhilip G Stevenson
Apr 29, 2005·Journal of Virology·B Karsten TischerNikolaus Osterrieder
Aug 17, 2012·Journal of Virology·Céline LétéLaurent Gillet
Sep 21, 2012·Journal of Virology·Kevin MaringerGillian Elliott
May 9, 2000·Journal of Virology·P DietzT C Mettenleiter
Dec 17, 2014·Journal of Virology·Imane El Kasmi, Roger Lippé

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