Glycosaminoglycans and periodontal disease: analysis of GCF by safranin O

Journal of Periodontology
W V GiannobileC M Cobb

Abstract

The purpose of this study was to quantify glycosaminoglycans (GAG) released into the gingival crevicular fluid (GCF) during health, gingivitis, and adult periodontitis. The investigation tested the hypothesis that increased amounts of GAG can be measured in GCF associated with gingivitis and adult periodontitis as compared to health. An individual patient's sampling sites were assigned to either a health (control) group or 1 of 3 experimental groups, gingivitis, periodontal "maintenance" (perio-M), or periodontal "non-maintenance" (perio-NM) according to standard clinical criteria of pocket probing depth, bleeding on probing, and radiographic evidence of bone loss. The perio-M group was defined as a periodontal patient who had received a dental prophylaxis and/or root planning within 6 months prior to GCF collection. The perio-NM group had received no periodontal therapy during the previous 6 months. Subsequent to air-drying and isolation, GCF was collected by a microcapillary pipette held at the gingival margin. All fluid samples were digested overnight at 37 degrees C with 25 micrograms of papain and analyzed for GAG content using a chondroitin-4-sulfate standard. Data generated from safranin "O" dye binding assays of GAG rev...Continue Reading

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Citations

Oct 21, 2011·Clinical Oral Investigations·Karim M Fawzy El-SayedMona S Darhous
Feb 3, 2015·Journal of Clinical Periodontology·Kristina BertlAndreas Stavropoulos
Mar 8, 2003·Journal of Clinical Periodontology·H JentschR Göcke
Dec 3, 2014·Journal of Periodontal Research·A H S HuynhI B Darby
Nov 1, 1996·Annals of Periodontology·G C Armitage
Dec 14, 2002·Journal of Periodontology·Laurie K McCauley, Rahime M Nohutcu
Sep 30, 2003·Journal of Periodontology·Gary C Armitage, UNKNOWN Research, Science and Therapy Committee of the American Academy of Periodontology
Mar 27, 2003·Periodontology 2000·William V GiannobileDavid P Sarment
Aug 1, 2003·Journal of Periodontology·UNKNOWN Research, Science and Therapy Committee
Dec 22, 2009·Journal of Proteome Research·Luan H NgoEric C Reynolds

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