PMID: 8445004Feb 5, 1993Paper

Glycosaminoglycans and proteins: different behaviours in high-performance size-exclusion chromatography

Journal of Chromatography
Nicola Volpi, L Bolognani

Abstract

The influence of the conformation of globular proteins and glycosaminoglycans in high-performance size-exclusion chromatography (HPSEC) was studied. Glycosaminoglycans (heparin, chondroitin sulphate and dermatan sulphate) with different primary structures, sulphate-to-carboxyl ratios and physico-chemical properties were extracted and purified. Their physico-chemical properties and purity were evaluated by several analytical techniques. Glycosaminoglycans with different relative molecular masses (M(r)) were prepared by a chemical depolymerization process. These heteropolysaccharides were evaluated by HPSEC and compared with globular proteins of known relative molecular mass. The two third-degree polynomial regression curves for proteins and glycosaminoglycans have different coefficients and the columns present different exclusion limits. In particular, under the experimental conditions, the M(r) exclusion limits for high M(r) are 44,000 for glycosaminoglycans and 240,000 for globular proteins. In contrast, the behaviours of these two classes of macromolecules are similar for lower M(r). In fact, the two third-degree polynomial curves show the same regression below about M(r) = 1000. The behaviour in HPSEC is discussed in relatio...Continue Reading

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Citations

Jan 18, 1995·Biochimica Et Biophysica Acta·N VolpiT Venturelli
Dec 9, 1994·Journal of Chromatography. B, Biomedical Applications·J G JoyceE D Lehman
Jul 21, 1995·Journal of Chromatography. B, Biomedical Applications·N VolpiT Venturelli
Jul 17, 2007·Journal of Pharmaceutical Sciences·Nicola Volpi
Mar 27, 2010·Biologicals : Journal of the International Association of Biological Standardization·B MulloyE Gray
Mar 9, 2011·Journal of Pharmaceutical and Biomedical Analysis·Keita Yamada, Kazuaki Kakehi
Dec 17, 2015·Pharmacological Reviews·Barbara MulloyClive P Page
Dec 29, 2020·Journal of Pharmaceutical and Biomedical Analysis·Nicola VolpiVeronica Mantovani
May 10, 2005·Biomacromolecules·Elisa LuppiNicola Volpi

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