Glycosaminoglycans and proteoglycans from different zones of the porcine knee meniscus

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
T NakanoP G Scott

Abstract

Medial and lateral knee menisci were obtained from 20-week-old pigs, dissected into three zones of equal width, and analyzed for collagen and glycosaminoglycan content and for types of glycosaminoglycan and proteoglycan. The thin inner zones contained about 76% collagen and 8% glycosaminoglycan (by dry weight) and the outer zones, 93% collagen and 2% glycosaminoglycan. The most abundant glycosaminoglycan in all zones was chondroitin sulphate, accounting for about 80% of total glycosaminoglycan in the inner zones and 50-56% in the outer zones. Dermatan sulphate was the second most abundant glycosaminoglycan, present relative to chondroitin sulphate in a ratio of about 1:5-6 in the inner zones and 1:1.5 in the outer zones. Hyaluronic acid accounted for 4-5% of total glycosaminoglycan content in the inner zones and 10% in the outer zones. All compositional parameters for the middle zones were between those for the inner and outer zones. There were no statistically significant differences in composition between medial and lateral menisci. Proteoglycans were extracted and separated into two groups (large and small proteoglycans) by gel chromatography and were further characterized by gel electrophoresis. The large proteoglycans stai...Continue Reading

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