Glycosaminoglycans in embryonic mouse teeth and the dissociated dental constituents

Differentiation; Research in Biological Diversity
E C Lau, J V Ruch

Abstract

The nature, amounts and distribution of glycosaminoglycans (GAG) before and during odontoblast terminal differentiation were studied. GAG have been isolated from intact mouse tooth germs and from dissociated dental epithelia and dental papillae after labeling with [3H]glucosamine or 35SO2-(4) asd precursor. The kinds and relative amounts of 3H-labeled GAG were analyzed by chromatography on a DEAE-cellulose column and cellulose thin-layer sheets. The amounts of individual GAG relative to total GAG were determined from the elution profiles, whereas their nature was identified by the selective removal of chromatographic peaks after enzymatic or chemical degradation. We found hyaluronate and probably a minute quantity of heparan sulfate in the dental epithelium, while hyaluronate, heparan sulfate, and chondroitin sulfate were the main types of GAG in the dental papilla. The chondroitin sulfate recovered was further fractionated by cellulose thin-layer chromatography into two isomers, namely chondroitin-2-sulfate (the major component) and chondroitin-6-sulfate. Changes in the elution profile from DEAE-cellulose chromatography of tooth GAG extracted from different developmental stages suggest that modifications of GAG occur during od...Continue Reading

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Citations

Mar 9, 2004·Archives of Oral Biology·S FelszeghyS Nielsen
Jan 1, 1985·Differentiation; Research in Biological Diversity·H LesotJ V Ruch
Jun 1, 1989·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Lindsay Heath, Peter Thorogood
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Jul 1, 1987·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Colin A B JahodaPhilippe Sengel
Apr 1, 1985·Journal of Dental Research·J V Ruch
Sep 29, 2020·Frontiers in Physiology·Natalia SánchezMarcia Gaete

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