Glycosaminoglycans in ewe reproductive tracts and their influence on acrosome reactions in bovine spermatozoa in vitro

Journal of Animal Science
C N LeeR L Ax

Abstract

Glycosaminoglycans (GAG) promote acrosome reactions (AR) in bovine and rabbit spermatozoa in vitro. Female bovine reproductive tract secretions contained GAG and the concentrations and composition of those GAG varied with different regions and stages of the estrous cycle. This study was designed to evaluate the types of GAG found in reproductive tract secretions of ewes at different stages of the estrous cycle and the ability of the secretions to promote AR. Ewes (n = 48) were slaughtered at 0, 12, 24, 36, 72 and 144 h following observations of standing estrus. Reproductive tracts were flushed with a modified Tyrode's medium (TALP: 7 ml) not containing calcium. Concentrations and composition of GAG in the tract were determined by high performance liquid chromatography. Concentrations of GAG decreased anterior to the cervix: 2.28 to .74 mg/100 mg protein (P less than .05). Increases in heparin-like GAG during the estrual phase and chondroitin sulfate GAG during the luteal phase were noted. Bovine sperm were incubated in tract flushings standardized to contain 4 micrograms/ml of GAG and supplemented with calcium. Sperm incubated in estrual flushings for 9 h exhibited a higher incidence of AR than those incubated in luteal flushin...Continue Reading

Citations

Jan 1, 1988·Journal of Dairy Science·D J Miller, R L Ax
Jul 20, 2007·Zygote : the Biology of Gametes and Early Embryos·A-S BergqvistH Rodríguez-Martínez
May 1, 1994·The Journal of Experimental Zoology·P WillisR A Fayrer-Hosken
Jul 1, 1987·Journal of Dairy Science·R L Ax, R W Lenz
Jun 10, 2004·The Journal of Veterinary Medical Science·Eiichi KawakamiToshihiko Tsutsui
Jun 1, 1990·Molecular Reproduction and Development·D J Miller, R L Ax
Nov 1, 1989·The Journal of Experimental Zoology·R R HandrowJ J Parrish
Nov 1, 1995·Andrologia·E Töpfer-PetersenF Sinowatz
Apr 3, 1998·The Journal of Veterinary Medical Science·E KawakamiT Tsutsui
Apr 12, 2005·Reproduction in Domestic Animals = Zuchthygiene·K LukoseviciuteA Januskauskas
Feb 18, 1998·Archives of Andrology·M L Sánchez-VázquezN M Delgado
Feb 27, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Neil Borg, Michael Holland
Jul 11, 2006·Zygote : the Biology of Gametes and Early Embryos·Ann-Sofi BergqvistHeriberto Rodríguez-Martínez
Jan 28, 2012·Reproduction, Fertility, and Development·Claire M Kershaw-YoungW M C Maxwell
Aug 16, 2005·Animal Reproduction Science·Ann-Sofi Bergqvist, Heriberto Rodríguez-Martínez
Nov 28, 2006·Biochemical and Biophysical Research Communications·Visesato MorTapati Chatterjee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.