PMID: 20657722Jul 27, 2010Paper

Glycosaminoglycans in Human and Bovine Serum: Detection of Twenty-Four Heparan Sulfate and Chondroitin Sulfate Motifs Including a Novel Sialic Acid-modified Chondroitin Sulfate Linkage Hexasaccharide.

Glycobiology Insights
Hong LuLijuan Zhang

Abstract

Heterogeneous heparan sulfate and chondroitin sulfate glycosaminoglycan (GAG) polysaccharides are important components of blood circulation. Changes in GAG quantity and structure in blood have been indicated in cancers and other human diseases. However, GAG quantities and structures have not been fully characterized due to lack of robust and sensitive analytical tools. To develop such tools, we isolated GAGs from serum and plasma. We employed liquid chromatography (LC) for GAG quantification and LC/mass spectrometry (MS) for GAG structural analysis. Twenty-four heparan and chondroitin sulfate motifs were identified, including linkage hexasaccharides, repeating disaccharide compositions, reducing, and non-reducing end mono-, di-, tri-, and tetrasaccharide structures. Disaccharides were detectable at picomolar level without radiolabeling or derivitization, so only a few ml of human and fetal bovine serum was required for this study. The detection of different reducing end structures distinct from GAG linkage hexasaccharides revealed that free GAG chains generated by GAG degradation enzymes co-existed with proteoglycans in serum. In addition, a novel sialic acid-modified linkage hexasaccharide was found conjugated to bikunin, the ...Continue Reading

Citations

Jun 14, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Evgenia KarousouNikos K Karamanos
Oct 4, 2011·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Makoto NishioKazuto Nishio
Nov 15, 2014·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Chuan ChenXi-Le Zhao
Mar 21, 2015·IEEE Transactions on Neural Networks and Learning Systems·David Reverter ValeirasRyad Benosman
Aug 16, 2015·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Ruimao ZhangLei Zhang
Feb 17, 2015·Biochemical and Biophysical Research Communications·Katarzyna Winsz-SzczotkaKrystyna Olczyk
Jun 21, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jia Yan DuZheng Wei
Nov 4, 2016·Proteomics·Yasunobu YoshidaYasuro Shinohara
Feb 19, 2020·Journal of Cellular and Molecular Medicine·Ying LanLijuan Zhang

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