Jul 1, 1976

Glycosidases of molluscs. Purification and properties of alpha-L-fucosidase from Chamelea gallina L

European Journal of Biochemistry
A Reglero, J A Cabezas

Abstract

An alpha-L-fucosidase had been purified approximately 300-fold from the liver (hepatopancreas) of the marine mollusc Chamelea gallina L. (= Venus gallina L.). During the different steps of the purification procedure it was difficult to remove the contaminant N-acetylglucosaminidase activity; but, after electrofocusing, a final preparation free of this and other glycosidades present in the crude extract was obtained. The purified enzyme has a broad specificity; it hydrolyzes p-nitrophenyl alpha-L-fucoside and natural substrates such as oligosaccharides containing fucosidic residues with alpha 1--2, alpha 1--3 and alpha 1--4 linkages; also it hydrolyzes fucose-containing glycopeptides, such as thyroglobulin glycopeptide, and glycoproteins as procine submaxillary mucin (previously rendered free of sialic acid). The enzyme has a pH optimum of 5.2 +/- 0.2, with a Km of 7 X 10(-5) M using p-nitrophenyl L-fucoside as substrate. It is inhibited by Hg2+ and some sugars, and activated by CN-, Zn2+, Ca2+ and EDTA. It shows two peaks by isoelectric focusing (at 6.3 and 6.6). The molecular weight of the alpha-L-fucosidase by gel filtration was over 2000000.

  • References18
  • Citations17

References

  • References18
  • Citations17

Citations

Mentioned in this Paper

Cations, Divalent
Mollusca
Isocyanides
Disaccharidases
Edetic Acid, Sodium Salt
FUCA1
Drug Stability
Fucosidase
Hydrogen-Ion Concentration
Enzyme Activation

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