PMID: 7458Jul 1, 1976

Glycosidases of molluscs. Purification and properties of alpha-L-fucosidase from Chamelea gallina L

European Journal of Biochemistry
A Reglero, J A Cabezas

Abstract

An alpha-L-fucosidase had been purified approximately 300-fold from the liver (hepatopancreas) of the marine mollusc Chamelea gallina L. (= Venus gallina L.). During the different steps of the purification procedure it was difficult to remove the contaminant N-acetylglucosaminidase activity; but, after electrofocusing, a final preparation free of this and other glycosidades present in the crude extract was obtained. The purified enzyme has a broad specificity; it hydrolyzes p-nitrophenyl alpha-L-fucoside and natural substrates such as oligosaccharides containing fucosidic residues with alpha 1--2, alpha 1--3 and alpha 1--4 linkages; also it hydrolyzes fucose-containing glycopeptides, such as thyroglobulin glycopeptide, and glycoproteins as procine submaxillary mucin (previously rendered free of sialic acid). The enzyme has a pH optimum of 5.2 +/- 0.2, with a Km of 7 X 10(-5) M using p-nitrophenyl L-fucoside as substrate. It is inhibited by Hg2+ and some sugars, and activated by CN-, Zn2+, Ca2+ and EDTA. It shows two peaks by isoelectric focusing (at 6.3 and 6.6). The molecular weight of the alpha-L-fucosidase by gel filtration was over 2000000.

References

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Citations

Jul 15, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·J SkrhaJ Páv
Jan 1, 1981·The International Journal of Biochemistry·A RegleroJ A Cabezas
Jan 1, 1990·The International Journal of Biochemistry·J Garcia-AlonsoJ A Cabezas
Aug 1, 1985·Molecular and Biochemical Parasitology·M L Rhoads
Jan 1, 1978·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·P CalvoJ A Cabezas
Jan 1, 1978·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E VillarJ A Cabezas
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S W Johnson, J A Alhadeff
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Sep 17, 2008·Clinical Biochemistry·Edimar Cristiano PereiraDulcineia Saes Parra Abdalla
Jun 20, 2015·International Journal of Endocrinology·Edimar Cristiano PereiraDulcineia Saes Parra Abdalla
Mar 31, 2018·Archives of Insect Biochemistry and Physiology·Natalia N PerrellaAdriana R Lopes
Jun 8, 2001·European Journal of Biochemistry·O J CorderoF J Rodríguez-Berrocal
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Jul 3, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yolanda Escamilla-LozanoAlma Cruz-Guerrero
Dec 24, 2018·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Talia B HeadLars Tomanek

Related Concepts

Cations, Divalent
Isocyanides
Disaccharidases
Drug Stability
Edetic Acid, Sodium Salt
Enzyme Activation
Fucosidase
Hydrogen-Ion Concentration
Liver
Mollusca

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