Glycosphingolipid composition of primary cultured human brain microvascular endothelial cells

Journal of Neuroscience Research
Takashi KandaHidehiro Mizusawa

Abstract

Glycosphingolipid (GSL) antigens have been considered to be involved in the pathogenesis of autoimmune neurologic disorders including multiple sclerosis. To establish the GSL pattern specific for endothelial cells forming blood-brain barrier (BBB), we established a method to yield sufficient quantities of highly purified human brain microvascular endothelial cells (HBMECs) and compared their GSL composition to that of human umbilical cord vein endothelial cells (HUVECs), as the representative of endothelial cells not forming BBB. The major gangliosides were GM3 and sialyl paragloboside (LM1), and the major neutral GSLs were lactosylceramide (LacCer), globotriaosylceramide (Gb3), and globoside (Gb4). Trace amounts of GM1, GD1a, GD1b, GT1b, and sulfoglucuronosyl paragloboside (SGPG) could be detected by the high performance thin layer chromatography-overlay method. SGPG was detected only at a nonconfluent state in an amount almost 1/30 that of in nonconfluent HUVECs. Conversely, GM3 and LM1 increased significantly after confluency. The amount of Gb3 in HBMECs was almost as twice that in HUVECs. The significance of these differences in GSL content between HBMECs and HUVECs and between confluent and nonconfluent states is obscure. ...Continue Reading

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Citations

Nov 29, 2012·PloS One·Ariane DesselleFrançois Paris
Jul 26, 2011·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Toshio Ariga
Jul 7, 2012·Cellular and Molecular Life Sciences : CMLS·Andreas BauwensJohannes Müthing
Jul 24, 2007·Molecular Genetics and Metabolism·Jin-Song ShenChristine R Kaneski
Sep 18, 2009·Annals of the New York Academy of Sciences·Tetsuo KubotaKazuo Umezawa
Dec 21, 2005·Biochemical and Biophysical Research Communications·Taro HinoHidehiro Mizusawa
Apr 7, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jean-Eric AlardChristophe Jamin
Sep 28, 2020·Analytica Chimica Acta·Rodell C Barrientos, Qibin Zhang

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