Glycosphingolipids and oxidative stress: evaluation of hydroxyl radical oxidation of galactosyl and lactosylceramides using mass spectrometry

Chemistry and Physics of Lipids
Daniela CoutoM R M Domingues

Abstract

Galactosylceramide (GalCer) and lactosylceramide (LacCer) are structural and signaling lipids, playing important roles in signal transduction and cell adhesion. They are especially abundant in the nervous system and in important components of the myelin sheath. Although neurodegenerative disorders are associated with increased oxidative stress and lipid oxidation, the connection between oxidative stress and glycosphingolipid modification has been scarcely addressed. In this study, we aimed to characterize the structural changes caused by the hydroxyl radical to GalCer and LacCer molecular species using electrospray ionization mass spectrometry (ESI-MS and MS/MS) and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS(n)). ESI-MS and LC-MS spectra of 24:1GalCer and 24:1LacCer after free radical oxidation showed the formation of new species, which were identified as keto, hydroxyl and hydroperoxy derivatives, arising from modification in the mono unsaturated fatty acyl chain. Formation of ceramide and oxidized ceramides was also observed as a result of 24:1GalCer and 24:1LacCer radical oxidation. 24:1GlcCer (glucosylceramide) was detected after LacCer oxidation, probably due to oxidative cleavage of lactosyl ...Continue Reading

References

May 10, 2001·Rapid Communications in Mass Spectrometry : RCM·P DominguesA J Ferrer-Correia
Nov 28, 2001·Immunology·D B Moody, G S Besra
Jul 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marcus S CookeJoseph Lunec
Nov 5, 2003·Journal of the American Society for Mass Spectrometry·Ana ReisPedro Domingues
Oct 22, 2004·Nature Reviews. Neuroscience·Luigi ZeccaRobert R Crichton
Mar 9, 2005·Chemistry and Physics of Lipids·Irina YurkovaOleg Shadyro
Jun 2, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ana ReisP Domingues
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Arundhati Jana, Kalipada Pahan
Dec 7, 2007·Biochimica Et Biophysica Acta·Francesco M Megli, Luciana Russo
Aug 2, 2008·Chemistry and Physics of Lipids·M Rosário M DominguesPedro Domingues
Mar 10, 2010·Journal of Lipid Research·You-Hai XuGregory A Grabowski
Oct 19, 2010·Journal of Neurochemistry·Louis J SparveroHülya Bayir
Dec 31, 2010·Rapid Communications in Mass Spectrometry : RCM·Elisabete MacielM Rosário M Domingues
Apr 15, 2011·Journal of Lipids·Wei-Ching HuangChiou-Feng Lin
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Elisabete MacielM Rosário M Domingues
Oct 18, 2011·Carbohydrate Research·Joana TudellaM Rosario M Domingues
Jun 12, 2012·Oxidative Medicine and Cellular Longevity·Sonia Gandhi, Andrey Y Abramov
Jun 20, 2012·Molecular Neurodegeneration·Cheng FangGary Banker
Dec 20, 2012·Cell Biochemistry and Biophysics·Cláudia SimõesM Rosário M Domingues
Jun 5, 2013·Current Neuropharmacology·Peter Kovacic, Ratnasamy Somanathan
Jul 23, 2013·Annals of Neurology·Simon HametnerHans Lassmann
Feb 12, 2014·Progress in Lipid Research·Bruno M CastroLiana C Silva
Oct 4, 2014·Rapid Communications in Mass Spectrometry : RCM·Deolinda SantinhaM Rosário M Domingues

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Citations

Nov 1, 2016·Journal of the American Society for Mass Spectrometry·Daniela CoutoPedro Domingues
Nov 27, 2019·Expert Reviews in Molecular Medicine·Jennifer Q J ZhangGopala K Rangan
Dec 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elena A SantalovaPavel S Dmitrenok
Aug 24, 2017·Journal of the American Society for Mass Spectrometry·Rodell C BarrientosQibin Zhang
Jun 13, 2021·Journal of Chromatography. a·Tomás Clive Barker-TejedaCoral Barbas

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