Glycosphingolipids of skeletal muscle: I. Subcellular distribution of neutral glycosphingolipids and gangliosides in rabbit skeletal muscle

Carbohydrate Research
J MüthingS Weber-Schürholz

Abstract

Membrane vesicles were prepared from rabbit skeletal muscle, separated by sucrose density gradient centrifugation and characterized by their specific marker enzymes, ligand binding, and ion flux activities. The fractions obtained (in the order of increasing density) were sarcolemma (SL), T-tubules (TT), sarcoplasmic reticulum (SR1 and SR2) and triads/mitochondria (Tr/M). Their glycosphingolipid compositions were analyzed by biochemical and immunochemical methods with specific antibodies (TLC immunostaining) and characteristic patterns were obtained from respective membrane fractions, expressed on a protein basis. Glucosylceramide, the main neutral glycosphingolipid of rabbit muscle, was found in SL and TT fractions, whereas SR and Tr/M vesicles lack this compound. Lactosylceramide was selectively recovered in the SR1 fraction. GM3(Neu5Ac), the main ganglioside in rabbit muscle, was found to account for 64% in the SL, 13% in the TT, 7% in the SR1, 3% in the SR2 and 13% in the Tr/M fractions. IV3Neu5Ac-nLcOse4Cer was mostly abundant in SL and decreased in the order SL > TT, Tr/M > SR1, SR2. IV6Neu5Ac-nLcOse4Cer was only detected in the SL and Tr/M fractions in noteworthy quantities. Ganglioseries gangliosides GM1, GD1a, GD1b and ...Continue Reading

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Citations

Feb 8, 2002·Archives of Biochemistry and Biophysics·Megumi Saito, Kiyoshi Sugiyama
May 27, 2005·Virchows Archiv : an International Journal of Pathology·H HůlkováM Elleder
Nov 2, 2012·Skeletal Muscle·Alessandro FanzaniEugenio Monti
Mar 10, 2010·Journal of Lipid Research·You-Hai XuGregory A Grabowski
Apr 17, 2012·Journal of Nutrition and Metabolism·John Janez MiklavcicMichael Thomas Clandinin
May 28, 2005·The Journal of Biological Chemistry·Marietta Flores-DíazMonica Thelestam
Oct 29, 2021·FEBS Open Bio·Laura Monturiol-GrossAlberto Alape-Girón

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