Glycosphingolipids of skeletal muscle: II. Modulation of Ca2(+)-flux in triad membranes by gangliosides

Carbohydrate Research
J MüthingS Weber-Schürholz

Abstract

Membrane vesicles of rabbit skeletal muscle were prepared and separated by sucrose density gradient centrifugation. The fractions obtained (in the order of increasing density) were sarcolemma (SL), T-tubules (TT), sarcoplasmic reticulum (SR1 and SR2) and triads/mitochondria (Tr/M) as characterized by their specific marker enzymes, ligand binding, and ion flux activities. The distribution of neutral glycosphingolipids and gangliosides in these membrane preparations has been documented in the preceding paper (J. Müthing, U. Maurer, U. Neumann, B. Kniep, and S. Weber-Schürholz, Carbohydr, Res., (1988) 135-145). GM3(Neu5Ac) is the dominant ganglioside, neolacto-series gangliosides are moderately expressed and ganglio-series gangliosides were found in minor quantities, however, all showing different qualitative and quantitative membrane-type specific patterns. The voltage dependent Ca(2+)-channels of skeletal muscle reside prevalently in the triad enriched membrane fractions deduced from highest binding capacity of 1,4-dihydropyridines. Calcium channel complexes of triads were reconstituted into unilamellar phospholipid vesicles of 400 nm defined size and the active 45Ca(2+)-uptake into intravesicular space was measured after incorp...Continue Reading

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Citations

Nov 1, 2011·Annals of Biomedical Engineering·Ruben T AlmarazKevin J Yarema
Apr 2, 2011·Antioxidants & Redox Signaling·Mariana N Nikolova-Karakashian, Michael B Reid
Nov 2, 2012·Skeletal Muscle·Alessandro FanzaniEugenio Monti
Apr 8, 2014·Acta histochemica·Mirca MariniEleonora Sgambati
Feb 24, 2015·Skeletal Muscle·Neha Singhal, Paul T Martin
Apr 17, 2012·Journal of Nutrition and Metabolism·John Janez MiklavcicMichael Thomas Clandinin
Oct 16, 2014·Journal of the American Society for Mass Spectrometry·Feifei ZhuDavid E Clemmer
Oct 22, 2014·SpringerPlus·Yosef Dror, Michael Hopp
Mar 23, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Paola RossiGiovanni Antonini

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