PMID: 6981424Sep 1, 1982Paper

Glycosyl ureides in ruminant nutrition. 1. Preparation and estimation of lactosyl urea and other glycosyl ureides

The British Journal of Nutrition
R J MerryA B McAllan

Abstract

1. Glucosyl urea, lactosyl urea and galactosyl urea were prepared from pure sugars and urea and their purity confirmed by determination of their melting points, specific rotations and by mass spectrometry. 2. Using whey as a lactose source, a range of conditions were examined for the preparation of lactosyl urea on a laboratory scale. Yields of 60% were achieved when lactose and urea (molar ratio, urea: lactose 0:6) were reacted for 15 h in sulphuric acid at pH 2.0 and a temperature of 70 degrees. 3. Methods of detection and estimation of all three ureides in whey preparations and ruminant digesta samples were developed. Two quantitative methods, one involving acid-hydrolysis, the other ion-exchange chromatography, were used. The latter method enabled determination of individual quantities of ureides in mixtures.

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Citations

Feb 1, 1986·Archiv für Tierernährung·R H Smith
Sep 1, 1982·The British Journal of Nutrition·O A Ikwuegbu, J D Sutton
Aug 4, 2011·Equine Veterinary Journal. Supplement·D G M SuttonS Love
Jan 1, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Karen P GeboesKristin Verbeke
Sep 27, 2003·Alimentary Pharmacology & Therapeutics·K P GeboesK Verbeke
Aug 29, 1997·Biochimica Et Biophysica Acta·F M RuemmeleM J Lentze

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