PMID: 3767256Jan 1, 1986Paper

Glycosylated haemoglobin: comparison of five different methods, including measurement on capillary blood samples

Annals of Clinical Biochemistry
J C MooreR C Turner

Abstract

Glycosylated haemoglobin was measured in venous blood samples and in blood collected in 'Unistep' bottles by isoelectric focusing (IEF), as the reference method, and by electroendosmosis (EEO), the thiobarbituric acid method (TBA), ion-exchange chromatography (IEC) and affinity chromatography (AC). Isoelectric focusing, electroendosmosis and thiobarbituric acid gave similar results. Affinity chromatography gave lower results than isoelectric focusing for normal values but similar results for diabetics. Ion-exchange chromatography gave 24% lower results than isoelectric focusing across the range. Using Unistep collected blood samples and comparing multiple samples from the same patient, electroendosmosis gave the best results (coefficient of variation 4%) and thiobarbituric acid gave slightly less good precision that other methods. Re-use of affinity chromatography columns gave less good precision. Collection of blood samples into a Unistep bottle gave similar results to venous sample results. Storage of venous capillary blood samples in Unistep bottles over 1 week at 21 degrees C gave similar results to immediate assay. Electroendosmosis of blood samples in Unistep bottles gave stable results over 2 weeks. Home collection by a ...Continue Reading

References

Oct 15, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·I S Ross, P F Gibson
Mar 1, 1978·British Journal of Haematology·J JavidA Cerami
Mar 1, 1985·Annals of Clinical Biochemistry·R R HolmanR C Turner
Feb 18, 1971·The New England Journal of Medicine·L A TrivelliH T Lai

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Citations

Jan 1, 1987·Diabetic Medicine : a Journal of the British Diabetic Association·R R HolmanR C Turner
Jan 1, 1990·Archives of Disease in Childhood·S Bloomfield, J W Farquhar
Jan 1, 1990·Journal of Clinical Laboratory Analysis·D A Armbruster, L C Hawes
Nov 1, 1986·Annals of Clinical Biochemistry·M P van Dieijen-VisserP J Brombacher
Jan 1, 1987·Annals of Clinical Biochemistry·G PetranyiK G Alberti
Mar 1, 1989·Annals of Clinical Biochemistry·G A Maguire, S J Standing
May 1, 1987·Annals of Clinical Biochemistry·A I AlayashM T Wilson

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