Glycosylated hemoglobin in human and animal red cells. Role of glucose permeability
We have compared red cells from man and selected animals in order to determine the effect of glucose permeability on nonenzymatic glycosylation of hemoglobin. Glucose permeability was highest in the primates (human, baboon, rhesus monkey), lower in dogs and rabbits, and nearly zero in pigs. Glycosylation of hemoglobin was measured by three independent methods: cation-exchange chromatography on Bio-Rex 70 (Bio-Rad, Inc., Richmond, California), agar gel electrophoresis, and affinity chromatography. The colorimetric thiobarbituric acid test did not provide valid data on animal hemolysates. However, this test was useful for identifying glycosylated hemoglobin (HbA1c) components isolated on Bio-Rex chromatography. In all animals tested, levels of HbA1c (from Bio-Rex chromatography) and total glycosylated hemoglobin (from affinity chromatography) correlated well with glucose exposure, the product of intracellular glucose concentration, and red cell life span. These results indicate that nonenzymatic glycosylation of hemoglobin in mammals is determined by three major variables: mean plasma glucose concentration, red cell life span, and red cell glucose permeability.
Specific radioimmunoassay of glucitol-lysine--application to lens proteins in streptozotocin-diabetic rats
In vivo measurement of subcutaneous glucose concentrations with an enzymatic glucose sensor and a wick method
Differential expression of fructosyllysine-specific receptors on monocytes and macrophages and possible pathophysiological significance
Dominant-negative mutant hepatocyte nuclear factor 1alpha induces diabetes in transgenic-cloned pigs
Associations among milk production traits and glycosylated haemoglobin in dairy cattle; importance of lactose synthesis potential
Glycated haemoglobin in various pathological conditions: investigations based on a new, fully automated method
Effect of nicotinic acid-induced insulin resistance on pancreatic B cell function in normal and streptozocin-treated baboons
Independent association of HbA1c and nonalcoholic fatty liver disease in an elderly Chinese population
Nonvirally modified autologous primary hepatocytes correct diabetes and prevent target organ injury in a large preclinical model
Body mass index negatively influences glycated albumin, but not glycated hemoglobin, in diabetic patients
Evidence for interindividual heterogeneity in the glucose gradient across the human red blood cell membrane and its relationship to hemoglobin glycation
Determination of glycated hemoglobins in the rat: comparison between two different chromatographic methods and application in experimental diabetology
Application of isoelectric focusing in immobilized pH gradients to the study of acetaldehyde-modified hemoglobin
Labile A1C is inversely correlated with the hemoglobin glycation index in children with type 1 diabetes
Hemoglobin glycation index: a robust measure of hemoglobin A1c bias in pediatric type 1 diabetes patients
A study of the effect of diet on glycosylated haemoglobin and albumin levels and glucose tolerance in normal subjects
Distribution of HbA(1c) levels for children and young adults in the U.S.: Third National Health and Nutrition Examination Survey
Tissue distribution and evolution of fructosamine 3-kinase and fructosamine 3-kinase-related protein.
Oxidized LDL and Fructosamine Associated with Severity of Coronary Artery Atherosclerosis in Insulin Resistant Pigs Fed a High Fat/High NaCl Diet
Marked increase in rat red blood cell membrane protein glycosylation by one-month treatment with a cafeteria diet
Racial disparity in HbA1c persists when fructosamine is used as a surrogate for mean blood glucose in youth with type 1 diabetes
Glycosylated haemoglobin in the fetus: chemistry, laboratory measurements and future clinical implications
Swine models of type 2 diabetes mellitus: insulin resistance, glucose tolerance, and cardiovascular complications
Characterization of Insulin-Secreting Porcine Bone Marrow Stromal Cells Ex Vivo and Autologous Cell Therapy In Vivo
Physiologic Concepts That May Revise the Interpretation and Implications of HbA1C in Clinical Medicine: An American Perspective
Validation and determination of a reference interval for canine HbA1c using an immunoturbidimetric assay
A labile form of hemoglobin A1c is higher in African-American youth with type 1 diabetes compared to Caucasian patients at similar glucose levels
Simultaneous measurements of myocardial glucose metabolism and extracellular volumes with hybrid PET/MRI using concurrent injections of Gd-DTPA and [18F]FDG.
American Diabetes Association Journals
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