Glycosylated superparamagnetic nanoparticle gradients for osteochondral tissue engineering.

Biomaterials
Chunching LiMolly M Stevens

Abstract

In developmental biology, gradients of bioactive signals direct the formation of structural transitions in tissue that are key to physiological function. Failure to reproduce these native features in an in vitro setting can severely limit the success of bioengineered tissue constructs. In this report, we introduce a facile and rapid platform that uses magnetic field alignment of glycosylated superparamagnetic iron oxide nanoparticles, pre-loaded with growth factors, to pattern biochemical gradients into a range of biomaterial systems. Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage. Interestingly, the smooth gradients in growth factor concentration gave rise to biologically-relevant, emergent structural features, including a tidemark transition demarcating mineralized and non-mineralized tissue and an osteochondral interface rich in hypertrophic chondrocytes. This platform technology offers great versatility and provides an exciting new opportunity for overcoming a range of interfacial tissue engineering challenges.

Citations

Jan 23, 2019·Lab on a Chip·James P K ArmstrongMolly M Stevens
Mar 8, 2019·Advanced Materials·Chunching LiMolly M Stevens
Dec 12, 2019·Advanced Materials·Vítor M GasparJoão F Mano
Feb 9, 2020·Medicina·Eugen Sebastian GrădinaruAlina Gabriela Mihaela Grigore
Mar 27, 2020·Frontiers in Chemistry·Zhongyang LiuPeifu Tang
Oct 20, 2020·Advanced Materials·Hannah M ZlotnickRobert L Mauck
Nov 13, 2020·Tissue Engineering. Part B, Reviews·Wendy BalestriYvonne Reinwald
Jul 12, 2020·Trends in Biotechnology·Chunching LiMolly M Stevens
Jan 23, 2021·International Journal of Molecular Sciences·Érica Resende OliveiraAmin Shavandi
Mar 18, 2021·Bone Research·Jinfeng LiaoZhiyong Qian
May 30, 2020·Chemical Reviews·Matthew L BedellAntonios G Mikos
Sep 14, 2021·ACS Biomaterials Science & Engineering·Adriana Maria EnderTanja Weil
Dec 24, 2021·Journal of Materials Science. Materials in Medicine·Shan LiWenjun Wang

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Methods Mentioned

BETA
Protein Assay
ELISA
PCR
biopsy
dynamic light scattering
glycosylation
enzyme-linked immunosorbent assay
Raman microscopy

Software Mentioned

KinetDs
rms
FIJI
Meeker , Finite Element Method Magnetics ,
R
Origin
Wintest
Nanoparticle Tracking Analysis

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