Glycosylation enhances oxygen radical-induced modifications and decreases acetylhydrolase activity of human low density lipoprotein.

Basic Research in Cardiology
C NapoliG Ambrosio

Abstract

Posttranslational nonenzymatic glycosylation of native low-density lipoprotein (n-LDL) occurs both in vitro and in vivo in diabetic patients. Glycosylated LDL (glc-LDL) behave similarly to oxidized LDL in some respects. In fact, unlike n-LDL, uptake the glc-LDL can occur in part by the "scavenger" receptor(s), as also demonstrated for oxidized LDL. The enzyme acetylhydrolase, carried by LDL, catabolizes platelet activating factor (PAF). This enzymatic activity is inhibited in oxidized LDL. However, it is unknown whether glc-LDL have reduced acetylhydrolase activity. The first aim of the study was to investigate whether glc-LDL were more susceptible than n-LDL to oxidative modification, and which different oxygen radical species were involved in the phenomenon. Moreover, in order to investigate whether glycosylation may affect acetylhydrolase, we also measured this enzymatic activity in both n- and glc-LDL. In vitro glc-LDL and n-LDL were exposed to the oxidants xanthine/xanthine oxidase (X/XO; 2 mM and 100 mU/ml, respectively), or CuSO4 (10 microM) for 18 hs at 37 degrees C. Parallel experiments were done in the presence of the superoxide radical scavenger superoxide dismutase (SOD; 330 U/ml), the hydrogen peroxide scavenger ca...Continue Reading

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Citations

Oct 24, 2002·Journal of the American College of Cardiology·Claudio NapoliLouis J Ignarro
Feb 20, 2016·Biochemical and Biophysical Research Communications·Nicoleta AlexandruAdriana Georgescu
Mar 23, 2004·Annals of the New York Academy of Sciences·Filomena de NigrisClaudio Napoli
Nov 30, 2005·Atherosclerosis·A C KalioraH Schmidt
Feb 15, 2012·Journal of Cellular Physiology·Filomena de NigrisClaudio Napoli
Sep 23, 2020·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Ahmad Khusairi AzemiAida Hanum Ghulam Rasool
Sep 18, 2007·Cell Biochemistry and Biophysics·Sudesh VasdevPawan Singal
Jan 24, 2021·Cardiovascular Research·Yufeng YaoQing K Wang
Mar 24, 2021·Journal of Diabetes and Its Complications·Amera HalabiThomas H Marwick
Jun 3, 2021·Journal of Cardiovascular Development and Disease·Hazel AberdeenEmmanuel D Williams
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nikola GligorijevićTanja Ćirković Veličković
Jul 6, 2010·Archives of Biochemistry and Biophysics·Paola PalozzaAssunta Catalano

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