PMID: 7535755Mar 29, 1995Paper

Glycosylation of a novel member of the immunoglobulin gene superfamily expressed in rat carcinoma cell lines

International Journal of Cancer. Journal International Du Cancer
C ChadéneauM G Denis

Abstract

MAb E4 recognizes a 66-kDa glycoprotein, pE4, which is a member of the immunoglobulin gene superfamily. This protein is expressed at the cell surface in rat colon and mammary carcinomas, but only in trace amounts in normal adult rat tissues. Since expression of aberrant carbohydrate structures is often associated with malignant transformation, glycosylation of pE4 was analyzed. Reactivity of lectins with pE4 suggested the absence of N-acetylneuraminic acid, terminal galactose and O-linked glycan, and the presence of N-linked glycans. Tunicamycin treatment reduced the binding of MAb E4 to cancer cells suggesting that the E4 epitope is at least partially glycosylated. Digestions with neuraminidases, O-glycosidase and peptide-N-glycosidase F confirmed these results. Pronase treatment abolished the binding of MAb E4, indicating that E4 epitope involves not only a carbohydrate determinant but also a peptide moiety. Mild periodate oxidation abolished the binding of MAb E4, indicating that non-reducing terminus carbohydrates are part of the E4 epitope. Neutral sugar analysis revealed the absence of galactose and the presence of fucose. Since fucose is sensitive to periodate oxidation, this sugar could be the carbohydrate part of the d...Continue Reading

References

Apr 1, 1991·International Journal of Cancer. Journal International Du Cancer·C ChadeneauK Meflah
Jun 15, 1989·International Journal of Cancer. Journal International Du Cancer·A GirlingJ Taylor-Papadimitriou
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J P JohnsonG Riethmüller
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S Hoffman, G M Edelman

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Citations

Jun 18, 2015·Current Opinion in Virology·Marshall E KarpelTodd M Allen
Sep 11, 2010·FEBS Letters·Noa Stanietsky, Ofer Mandelboim
May 30, 2006·Advances in Immunology·Charles G DrakeDrew M Pardoll
Jan 15, 2015·Cancer Treatment Reviews·F MassariG Tortora
Jun 6, 2018·Frontiers in Immunology·Hristo GeorgievGünter Bernhardt
Dec 11, 1996·International Journal of Cancer. Journal International Du Cancer·C ChadéneauM G Denis

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