Glycosylation of ascites-derived exosomal CD133: a potential prognostic biomarker in patients with advanced pancreatic cancer

Medical Molecular Morphology
Takahiko SakaueTakuji Torimura

Abstract

Cancer cells surviving in ascites exhibit cancer stem cell (CSC)-like features. This study analyzed the expression of the CSC marker CD133 in the ascites-derived exosomes obtained from patients with unresectable pancreatic cancer. In addition, inverse correlation of CD133 expression with prognosis was examined. Of the 133 consecutive patients, 19 patients were enrolled in the study. Exosomes derived from the malignant ascites demonstrated higher density and wider variation in size than those from non-malignant ascites. Western blot revealed enhanced expression of CD133 in exosomes obtained from patients with pancreatic cancer compared to those obtained from patients with gastric cancer or liver cirrhosis. A xenograft mouse model with malignant ascites was established by intraperitoneal inoculation of human pancreatic cancer cells in nude mice. Results obtained from the human study were reproduced in the mouse model. Statistically significant equilateral correlation was identified between the band intensity of CD133 in western blot and overall survival of patients. Lectin microarray analyses revealed glycosylation of CD133 by sialic acids as the major glycosylation among diverse others responsible for the glycosylation of exosom...Continue Reading

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Citations

Apr 7, 2019·Medical Molecular Morphology·Christine A FargeasDenis Corbeil
Oct 30, 2020·International Journal of Nanomedicine·Victor C Kok, Cheng-Chia Yu
Feb 27, 2021·Analytical Chemistry·He YanYong Zeng
Apr 24, 2021·Frontiers in Oncology·Thahomina Khan, Horacio Cabral
May 3, 2021·Molecular Immunology·Janaina Macedo-da-SilvaGiuseppe Palmisano
Jun 5, 2021·Advanced Drug Delivery Reviews·Ana ŠpilakChrista Noehammer
Aug 15, 2021·International Journal of Biological Macromolecules·Guanwen GaoXiao Zhu
Nov 2, 2021·Frontiers in Cell and Developmental Biology·Shu-Ya LiuQing-Lian Wen

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