PMID: 7543137Aug 1, 1995Paper

Glycosylation of CD44 negatively regulates its recognition of hyaluronan

The Journal of Experimental Medicine
S KatohP W Kincade

Abstract

Although CD44 is expressed on a wide variety of cell types, few of them use it to recognize the ligand hyaluronan (HA). A glycosylation-defective clone of Chinese hamster ovary cells (Lec 8) bound HA, demonstrating that complete processing of glycoproteins with addition of a full complement of sialic acid is not required. On the contrary, subsequent findings revealed that complex sugars on CD44 can actually inhibit ligand recognition. Two subclones of wild-type Chinese hamster ovary cells with similar amounts of surface CD44 were isolated on the basis of HA binding and found to differ with respect to CD44 size as well as staining with fluorescent lectins. Treatment of the nonbinding clone with tunicamycin reduced the size of the protein and allowed the cells to recognize HA via CD44. This function was also induced by treatment with deglycosylating enzymes (either a mixture of endoglycosidase F and N-glycosidase F or neuraminidase alone). A possible role for glycosylation in regulation of adhesion was then sought with a series of normal and transformed murine cells. Disruption of glycosylation or treatment with deglycosylating enzymes did not induce ligand binding in an interleukin 7-dependent pre-B cell line, and splenic B cell...Continue Reading

References

Aug 1, 1992·The Journal of Cell Biology·L ThomasI Stamenkovic
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B ArdmanD E Staunton
Oct 18, 1991·Cell·M BevilacquaR McEver
Feb 1, 1992·The Journal of Cell Biology·S Jalkanen, M Jalkanen
Jan 25, 1992·Proceedings of the National Academy of Sciences of the United States of America·K IshiharaP W Kincade
Jul 1, 1990·The Journal of Experimental Medicine·K MiyakeP W Kincade
Dec 1, 1990·The Journal of Cell Biology·D D TrueS D Rosen
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S Hoffman, G M Edelman
Jul 1, 1994·The Journal of Experimental Medicine·A BartolazziI Stamenkovic
Apr 1, 1994·International Immunology·N Toyama-Sorimachi, M Miyasaka
Nov 1, 1994·Immunological Investigations·J M GuthridgeD A Cohen
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·T KaishoT Hirano
Jul 1, 1993·The Journal of Cell Biology·R J PeachA Aruffo

❮ Previous
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Citations

Mar 24, 1999·Journal of Cellular Biochemistry·L L SmithC M Giachelli
Jan 25, 2011·Annals of Hematology·Magdalena Katharina HertweckKarl-Anton Kreuzer
Jun 23, 1999·Experimental Hematology·S GhaffariC Eaves
Oct 23, 1997·Current Opinion in Cell Biology·P W KincadeL Hanson
Jun 12, 2003·The International Journal of Biochemistry & Cell Biology·Stuart George JonesAled Owain Phillips
Apr 28, 2001·Trends in Molecular Medicine·E Puré, C A Cuff
Nov 3, 2012·The Journal of Biological Chemistry·Cuixia YangFeng Gao
Dec 1, 1995·The Journal of Cell Biology·K L BennettA Aruffo
Mar 1, 1996·The Journal of Cell Biology·A BartolazziI Stamenkovic
Aug 1, 1996·The Journal of Cell Biology·K Oritani, P W Kincade
Dec 13, 2005·Clinical and Diagnostic Laboratory Immunology·M E Hunt, D R Brown
Jan 20, 1999·Molecular Pathology : MP·R J Sneath, D C Mangham
Aug 10, 1999·Molecular Pathology : MP·H F HsiehH J Harn
Apr 21, 2007·American Journal of Respiratory and Critical Care Medicine·Shigeki KatohMitsuomi Hirashima
Oct 6, 1997·American Journal of Respiratory Cell and Molecular Biology·P Teder, P Heldin
Jun 25, 1998·American Journal of Respiratory Cell and Molecular Biology·K MatsumotoB S Bochner
Feb 14, 1998·The Journal of Clinical Investigation·M MohamadzadehM Siegelman
Sep 3, 1998·The Journal of Clinical Investigation·J MollP Herrlich
Mar 11, 2000·The Journal of Clinical Investigation·M H SiegelmanP Estess
Jun 1, 2013·Journal of Inflammation·Katharine E BlackMaureen R Horton
Dec 1, 2006·The Journal of Reproduction and Development·Masaki YokooEimei Sato
Apr 8, 2010·International Journal of Oncology·Christopher S GondiJasti S Rao
Nov 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·C J DimitroffR Sackstein
Feb 27, 1999·Leukemia & Lymphoma·L J Bendall, D J Gottlieb
May 26, 1999·Leukemia & Lymphoma·R RistamäkiS Jalkanen
Feb 26, 1999·Leukemia & Lymphoma·K Oritani, P W Kincade
Aug 10, 2000·Immunological Investigations·S D Reid, A G Pockley

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