Glycosylation of flavonoids with a glycosyltransferase from Bacillus cereus

FEMS Microbiology Letters
Jae Hyung KoAhn Joong-Hoon

Abstract

Microbial glycosyltransferases can convert many small lipophilic compounds such as phenolics, terpenoids, cyanohydrins and alkaloids into glycons using uridine-diphosphate-activated sugars. The main chemical functions of glycosylation processes are stabilization, detoxification and solubilization of the substrates. The gene encoding the UDP-glycosyltransferase from Bacillus cereus, BcGT-1, was cloned by PCR and sequenced. BcGT-1 was expressed in Escherichia coli BL21 (DE3) with a his-tag and purified using a His-tag affinity column. BcGT-1 could use apigenin, genistein, kaempferol, luteolin, naringenin and quercetin as substrates and gave two reaction products. The enzyme preferentially glycosylated at the 3-hydroxyl group, but it could transfer a glucose group onto the 7-hydroxyl group when the 3-hydroxyl group was not available. The reaction products made by biotransformation of flavonoids with E. coli expressing BcGT-1 are similar to those produced with the purified recombinant enzyme. Thus, this work provides a method that might be useful for the biosynthesis of flavonoid glucosides and for the glycosylation of related compounds.

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Citations

Dec 14, 2011·Applied Microbiology and Biotechnology·Bong-Gyu KimJoong-Hoon Ahn
Apr 4, 2013·Applied Microbiology and Biotechnology·Hyeon Jeong KimJoong-Hoon Ahn
May 30, 2013·Applied Microbiology and Biotechnology·Myoung Chong SongYeo Joon Yoon
Oct 9, 2009·Molecules and Cells·Dinesh SimkhadaJae Kyung Sohng
Oct 18, 2012·Journal of Agricultural and Food Chemistry·Bong-Gyu KimJoong-Hoon Ahn
Apr 12, 2012·Applied and Environmental Microbiology·Jeong-A YoonJoong-Hoon Ahn
May 21, 2013·Applied and Environmental Microbiology·U RabauschW R Streit
Sep 10, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Yin-Cheng HsiehChun-Jung Chen
Mar 5, 2016·Journal of Industrial Microbiology & Biotechnology·Dae Gyun AnJoong-Hoon Ahn
Oct 29, 2010·Chembiochem : a European Journal of Chemical Biology·Bong-Gyu KimJoong-Hoon Ahn
Apr 10, 2013·Biotechnology and Bioengineering·Sailesh MallaJae Kyung Sohng
Feb 25, 2014·Journal of Biotechnology·Hyejin LeeJoong-Hoon Ahn
Oct 27, 2009·Microbiological Research·Yong-Mo KimJoong Su Kim
Mar 10, 2015·Applied Microbiology and Biotechnology·Bong Gyu KimJoong-Hoon Ahn
Feb 24, 2015·Biotechnology Advances·Frederik De BruynMarjan De Mey
Dec 18, 2014·Applied Microbiology and Biotechnology·So Yeon KimJoong-Hoon Ahn
Oct 6, 2017·PloS One·Monika DymarskaEdyta Kostrzewa-Susłow
Jan 18, 2018·Applied Microbiology and Biotechnology·Lars MilkeNicolai Kallscheuer
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Mar 1, 2019·Food Science and Biotechnology·Eun-Ryoung KimCheon-Seok Park
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Nov 4, 2020·Antimicrobial Agents and Chemotherapy·Punyawee DulyayangkulMatthew B Avison
Apr 3, 2020·Journal of Microbiology and Biotechnology·Puspalata BashyalJae Kyung Sohng

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