Glycosylation of the tandem repeat unit of the MUC2 polypeptide leading to the synthesis of the Tn antigen

Biochemical and Biophysical Research Communications
M InoueH Nakada

Abstract

A synthetic peptide corresponding to the human MUC2 tandem repeat domain containing 14 Thr residues was glycosylated in vitro using UDP-GalNAc and microsomal membranes of the colorectal cancer cell line, LS180. The products were fractionated by reverse phase HPLC, which gave seven glycopeptide fractions. Their molecular weights were estimated by matrix-assisted laser desorption/ionization mass spectrometry, the values obtained corresponding to glycopeptides containing from one to ten GalNAc residues. On solid phase radioimmunoassaying involving a monoclonal anti-Tn antibody (MLS128), it was found that the glycopeptides containing nine or ten GalNAc residues were strongly immunoreactive, whereas the glycopeptides containing less than six GalNAc residues were inactive, indicating that a cluster of GalNAc-Thr is essential for the Tn antigenicity.

References

Dec 1, 1990·Neuroendocrinology·D Wynick, S R Bloom
Oct 1, 1988·Japanese Journal of Cancer Research : Gann·S FukuiI Yamashina
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H NakadaI Yamashina
Aug 15, 1994·The Biochemical Journal·D J McCoolG G Forstner

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Citations

Apr 28, 2000·The Journal of Peptide Research : Official Journal of the American Peptide Society·S Vichier-GuerreD Cantacuzène
Dec 29, 2000·Bioscience, Biotechnology, and Biochemistry·H AshidaH Kumagai
Jul 12, 2005·Bioorganic & Medicinal Chemistry·Howard C Hang, Carolyn R Bertozzi
May 5, 1999·Journal of Mass Spectrometry : JMS·K AlvingJ Peter-Katalinic
Feb 6, 2002·Chembiochem : a European Journal of Chemical Biology·O Seitz

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